Km12l4

Manufactured by Korean Cell Line Bank

The KM12L4 is a laboratory equipment product offered by the Korean Cell Line Bank. It is designed for cell culture research purposes. The core function of the KM12L4 is to provide a controlled environment for the growth and maintenance of cell lines. The detailed specifications and intended use of this product are not available.

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Lab products found in correlation

3 protocols using km12l4

Colon Cancer Cell Line Culture

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Ten colon cancer cell lines (DLD1, HT29, HCT116, HCT15, SW620, LOVO, SW480, KM12C, KM12L4, and KM12SM) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in MEM, DMEM, RPMI1640, or L15 medium (Welgene, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Gibco), and maintained in a humidified incubator with 5% CO₂ at 37 °C.

Jang B.G., Kim H.S., Bae J.M., Kim W.H., Hyun C.L, & Kang G.H. (2020). Expression Profile and Prognostic Significance of EPHB3 in Colorectal Cancer. Biomolecules, 10(4), 602.

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Comprehensive CRC Cell Line Characterization

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Forty-eight human CRC cell lines (SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-479, SNU-503, SNU-769A, SNU-769B, SNU-796, SNU-977, SNU-1033, SNU-1040, SNU-1047, SNU-1181, SNU-1197A, SNU-1235, SNU-1411, SNU-1406, SNU-1544, SNU-1684, SNU-1746, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, Caco-2, COLO201, COLO205, COLO320, DLD1, HCT-8, HCT-15, HCT-116, HT-29, LoVo, LS174T, NCI-H716, SW-403, SW-480, SW-620, SW-1116, WiDr, KM12C, KM12SM, and KM12L4) were obtained from the Korean Cell Line Bank. All cell lines were maintained in RPMI1640 media with 10% fetal bovine serum at 37°C and 5% CO₂. All cell lines were tested and authenticated by Korean Cell Line Bank using DNA finger printing analysis.

Kim S.C., Hong C.W., Jang S.G., Kim Y.A., Yoo B.C., Shin Y.K., Jeong S.Y., Ku J.L, & Park J.G. (2018). Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential. Translational Oncology, 11(5), 1232-1243.

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Colorectal Cancer Cell Line Cultivation

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Twelve CRC cell lines (DLD1, HT29, SW620, HCT116, LoVo, KM12C, KM12SM, KM12L4, HCT116, LS174T, SW1116, and Colo205) were purchased from the Korean Cell Line Bank (https://cellbank.snu.ac.kr, Seoul, Korea). Cells were cultured in RPMI1640, MEM, DMEM, or L15 medium (Welgene, Daegu, Korea) at 37 °C in a humidified incubator with 5% CO2 according to the guidelines. All culture media contained 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA) and 1% penicillin/streptomycin (Gibco).

Jang B.G., Kim H.S., Bae J.M., Kim W.H., Kim H.U, & Kang G.H. (2020). SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancers. Scientific Reports, 10, 14591.

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