3 aminobenzoic acid ethyl ester methanesulfonate 222 ms 222

Undifferentiated sterlet testes samples were collected from 4 to 6 month-old individuals (∼10 cm in total length and ∼5 g in weight), differentiated gonad samples were collected from 16 to 18 month-old individuals (∼52 cm in total length and ∼200 g in weight) and maturing gonad samples were collected from 2 year-old individuals (∼69 cm in total length and ∼632 g in weight). Fish were sacrificed by deep anesthesia in 0.1% 3-aminobenzoic acid ethyl ester methanesulfonate-222 (MS-222) (Sigma, St. Louis, MO, United States).

Dabry’s sturgeon Acipenser dabryanus, used for optimization of germ cell culture conditions, were reared in the Taihu Station, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science. Sterlet Acipenser ruthenus used for germ cell transplantation were cultivated at the Faculty of Fisheries and Protection of Waters, University of South Bohemia. Gonads were collected from 22–26-month-old Dabry’s sturgeon (length ~92 cm; weight ~3.5 kg). Sterlet gonads were collected from 10–13-month-old specimens (~52 cm; ~520 g). The gonads were at maturity stage II: containing mostly spermatogonia or oogonia and previtellogenic oocytes. Deep anesthesia was induced by 0.05% 3-aminobenzoic acid ethyl ester methanesulfonate-222 (MS-222) (Sigma, St. Louis, MO, USA). Russian Sturgeon Acipenser gueldenstaedtii larvae obtained from combined eggs and sperm of three females and three males were used as recipients for cultured germ cells.