Tripyridyl s triazine

Manufactured by Merck Group

Sourced in United States

Tripyridyl-s-triazine is a chemical compound used in various laboratory applications. It is a chelating agent that forms stable complexes with metal ions. The core function of Tripyridyl-s-triazine is to act as a colorimetric reagent for the detection and quantification of iron(II) and iron(III) in aqueous solutions.

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2 protocols using tripyridyl s triazine

Characterizing Oregano Essential Oil

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Commercial O. vulgare essential oil (CO) was purchased from Botanicessence (Product of Spain) (Bangkok, Thailand). Carvacrol, α-tocopherol, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6, tripyridyl-s-triazine (TPTZ), hydrochloric acid (HCl), acetic acid (CH₃COOH), linoleic acid, hyaluronidase from bovine testes, sodium chloride (NaCl), collagenase from Clostridium histolyticum, N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), elastase from porcine pancreas, N-succinyl-Ala-Ala-Ala-p-nitroanilide (AAAVPN), sodium phosphate monobasic dihydrate (NaH₂PO₄.2H₂O), and sodium phosphate dibasic dihydrate (Na₂HPO₄.2H₂O) were purchased from Sigma-Aldrich (St. Louis, MO, USA); α-Ascorbic acid was purchased from Asia Pacific Specialty Chemicals Limited (New South Wales, Australia). Anhydrous sodium sulfate (Na₂SO₄), ferric chloride hexahydrate (FeCl₃.6H₂O), ferrous sulphate heptahydrate (FeSO₄.7H₂O), and ammonium thiocyanate (NH₄SCN) were purchased from Loba Chemie (Boisar, Tarapur, India). Bovine serum albumin was purchased from Merck (Darmstadt, Germany). Tricine was purchased from Bio-Rad Laboratories (Richmond, CA, USA). Sodium acetate trihydrate (CH₃COONa.3H₂O), calcium chloride dihydrate (CaCl₂.2H₂O), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), dichloromethane, methanol, and ethanol were analytical grade and purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand).

Laothaweerungsawat N., Sirithunyalug J, & Chaiyana W. (2020). Chemical Compositions and Anti-Skin-Ageing Activities of Origanum vulgare L. Essential Oil from Tropical and Mediterranean Region. Molecules, 25(5), 1101.

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Phytochemical Analysis and Antioxidant Assays

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All the chemicals, reagents and the solvents used in the assay protocols were of analytical grade. Ascorbic acid, sodium phosphate, phosphate buffer saline, ammonium molybdate, sodium citrate, DPPH, DMSO, Folin-Ciocalteu reagent, sodium acetate trihydrate, sodium hydroxide, and sodium citrate were obtained from Sigma Aldrich, USA. Other chemicals such as gallic acid, TRIS-HCl, sodium carbonate, sodium chloride, potassium ferricyanide, ferric chloride, tripyridyl-s-triazine (TPTZ), potassium dihydrogen phosphate, disodium hydrogen phosphate, and metaphosphoric acid were purchased from Sigma Aldrich. n-Hexane, EtOAc, MeOH, C₂H₅OH, H₂SO₄ of analytical grade and other chemicals were procured from Daejung Chemicals and Metals (Seoul, South Korea). Column chromatography was conducted by using silica gel (70–230 mesh) and LiChroprep RP-18 (40–63 μm; ODS silica gel) from Merck. Thin layer chromatography was achieved on glass backed precoated silica gel 60 F₂₅₄ plates (Merck). Resolution of the spots in TLC plates was checked by dipping the plate in a solution of 5% vanillin-H₂SO₄ in C₂H₅OH. Standards were procured from Sigma-Aldrich, USA. Instrumentation details such as optical rotation, FT-IR, ESI/FT mass and NMR spectra were given in the literature (Chung et al., 2014b (link)).

Chung I.M., Siddiqui N.A., Kim S.H., Nagella P., Khan A.A., Ali M, & Ahmad A. (2016). New constituents triterpene ester and sugar derivatives from Panax ginseng Meyer and their evaluation of antioxidant activities. Saudi Pharmaceutical Journal : SPJ, 25(5), 801-812.

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