Analyst software 1

Manufactured by AB Sciex

Sourced in United States, Germany

Analyst software 1.6.2 is a data processing and instrument control software developed by AB Sciex. The software provides tools for data acquisition, processing, and reporting for mass spectrometry-based analytical applications.

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Lab products found in correlation

Qtrap 5500, by AB Sciex (4 mentions) Multiquant software 3, by AB Sciex (3 mentions) Qtrap 6500, by AB Sciex (2 mentions) Qtrap 5500 mass spectrometer, by AB Sciex (2 mentions) Lichrospher 60 rp select b column, by Merck Group (2 mentions) Agilent 1100 series hplc system, by Agilent Technologies (2 mentions) Lc 20ab binary pump, by Shimadzu (1 mentions) Xterra ms c18 column, by Waters Corporation (1 mentions) Prism 7, by GraphPad (1 mentions) Acquity 1 class, by Waters Corporation (1 mentions) Chromeleon 6, by Thermo Fisher Scientific (1 mentions) Tripletof 5600, by AB Sciex (1 mentions) Cytidine, by Merck Group (1 mentions) Guanosine, by Merck Group (1 mentions) Adenosine, by Merck Group (1 mentions) Uridine, by Merck Group (1 mentions) Uflc nexera, by Shimadzu (1 mentions) Turbo ion spray source, by AB Sciex (1 mentions) Luna phenyl hexyl column, by Phenomenex (1 mentions) 1290 infinity uhplc system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Analyst 1.6.3 software (680 citations) Analyst software (148 citations) Analyst software version 1.6.2 (69 citations) Analyst TF software 1.7.1 (9 citations) Analyst software (V.1.6.2) (5 citations) Analyst software ver.1.5 (4 citations) SCIEX Analyst Work Station Software (Version 1.6.3) (3 citations) Sciex Analyst® Software (version 1.7.1) (2 citations) Analyst 1.5.2TM software (2 citations)

31 protocols using analyst software 1

Quantitative Analysis by LC-MS/MS

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Analyses were carried out by using the Prominence LC-20AB/SPD-20A liquid chromatography system that consisted of a LC-20AB binary pump equipped with an online degasser and an autosampler (Shimadzu Co., Kyoto, Japan). The chromatographic system was operated by using the Analyst software 1.5.1 (AB Sciex, Ltd., Framingham, MA, USA). Separations were conducted by using a 50 mm × 2.1 mm I.D. XTerra MS C18 column (Waters Co., Milford, MA, USA).
Detection was performed with an API3200 quadrupole mass spectrometry (AB Sciex, Ltd., Framingham, MA, USA), equipped with turbo ion spray interface, operated in the positive mode, and configured in multiple reaction monitoring mode. The mass spectrometry system was operated by using the Analyst software 1.5.1 (AB Sciex, Ltd., Framingham, MA, USA).

Ogawa-Morita T., Sano Y., Okano T., Fujii H., Tahara M., Yamaguchi M, & Minami H. (2017). Validation of a Liquid Chromatography-Tandem Mass Spectrometric Assay for Quantitative Analysis of Lenvatinib in Human Plasma. International Journal of Analytical Chemistry, 2017, 2341876.

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Quantifying Inhibitor Dose-Dependent Uptake

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The uptake experiments were conducted in technical triplicate and repeated independently 3 times with cells from different passages. For LC-MS/MS, instrument navigation and raw data processing and analysis were performed with Analyst software 1.6 (AB Sciex, Framingham, MA, USA). The lower limit of quantification (LLOQ) of mIBG was 0.1 nM, and the calibration curve was linear up to 8 μM. Non-linear regression was used to fit the data on GraphPad Prism 7.0 (GraphPad Software Inc., La Jolla, CA, USA) to generate graphs and calculate parameters. For the dose-dependent inhibition assay, the IC₅₀ values of inhibitors were calculated with the equation below:

v = Bottom + \frac{Top - Bottom}{1 + {(\frac{[I]}{{IC}_{50}})}^{h}}

where v is the rate of substrate uptake with inhibitor, Bottom is the baseline value at 100% inhibition, Top is the rate of substrate uptake without inhibitor, [I] is the inhibitor concentration, IC₅₀ is the half-maximal inhibitory concentration, and h is the Hill coefficient.

Tsang Y.P., López Quiñones A.J., Vieira L.S, & Wang J. (2023). Interaction of ALK Inhibitors with Polyspecific Organic Cation Transporters and the Impact of Substrate-Dependent Inhibition on the Prediction of Drug–Drug Interactions. Pharmaceutics, 15(9), 2312.

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Quantitative Analysis of Jasmonates by UPLC-MS/MS

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Jasmonates were measured by UPLC-ESI–MS/MS using a Waters Acquity I-Class ultra-high-performance liquid chromatography system (Milford, MA, USA) coupled to an AB Sciex 6500+ QTRAP^® tandem mass spectrometer (AB Sciex, Framingham, MA, USA), operated in the negative ionization mode. Extraction and chromatographic separation of plant material was carried out as described in [42 (link)] using dihydrojasmonic acid (dhJA, 5 ng), JA-Norvaline (JA-Nval, 5 ng) and [18O₂]OPDA (5 ng) as internal standards. Mass spectrometric analysis was performed under the following electrospray ionization (ESI) source conditions: ion spray voltage was −4000 V, curtain gas was kept at 30 psi and nebulizer gas (GS1) and drying gas (GS2) were adjusted to 50 and 80 psi, respectively, at a temperature of 600 °C. Detection was carried out by selected reaction monitoring (SRM) with a dwell time of 25 ms for each compound and the collision gas (CAD) gas set to 9 psi using the compound-dependent UPLC–MS/MS parameters listed in Supplementary Table S2. Data analysis was performed in Analyst software 1.6 from AB Sciex.

Thurow C., Krischke M., Mueller M.J, & Gatz C. (2020). Induction of Jasmonoyl-Isoleucine (JA-Ile)-Dependent JASMONATE ZIM-DOMAIN (JAZ) Genes in NaCl-Treated Arabidopsis thaliana Roots Can Occur at Very Low JA-Ile Levels and in the Absence of the JA/JA-Ile Transporter JAT1/AtABCG16. Plants, 9(12), 1635.

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Quantitative LC-MS/MS Analysis

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The composition of LC-MS/MS and the column used were the same as a previous publication [13 ]. The mobile phase A was composed of 2.0 mM ammonium acetate and 0.1% acetic acid in methanol, and mobile phase B was composed of 2.0 mM ammonium acetate and 0.1% acetic acid in water. The flow rate was 0.4 mL/min with the following elution gradient: 0.01-25 min: 5% B-1% B; 25–26 min: 1% B-5% B; 26–30 min: 5% B. Quantification was performed with Analyst Software 1.6 (AB SCIEX, USA).

Cui Y., Hou P., Li F., Liu Q., Qin S., Zhou G., Xu X., Si Y, & Guo S. (2017). Quercetin improves macrophage reverse cholesterol transport in apolipoprotein E-deficient mice fed a high-fat diet. Lipids in Health and Disease, 16, 9.

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SWATH-MS Quantitative Analysis Protocol

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For mass spectrometric analysis, the LC was coupled to a high-resolution quadrupole time of flight mass spectrometer (Triple TOF 5600+, Sciex, USA). Data-independent Sequential Window Acquisition of all Theoretical spectra (SWATH runs) technology based on MS2 quantification was used for quantitative measurements [14 (link)]. Peptides were fragmented in 35 fixed fragmentation windows of 20 Da in the range of 400–1100 Da with an accumulation time of 50 min in TOF–MS and 80 min in product ion mode. The nano-HPLC system was operated by Chromeleon 6.8 (Dionex, USA) and the MS by Analyst Software 1.6 (Sciex, USA).

Saleh M., Kumar G., Abdel-Baki A.A., Dkhil M.A., El-Matbouli M, & Al-Quraishy S. (2018). Quantitative shotgun proteomics distinguishes wound-healing biomarker signatures in common carp skin mucus in response to Ichthyophthirius multifiliis. Veterinary Research, 49, 37.

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Amino Acid Profiling of HAEC Cells

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HAEC were lysed and centrifuged for amino acid profiling. Additionally, cell culture supernatant was collected and centrifuged for 10 min at 17,000 × g. 200 µl of samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS system consisted of an Agilent 1260 Series binary pump (Agilent Technologies) and a triple quadrupole mass spectrometer 5500 QTRAP (Sciex). Calibration was done using the following standard substances: Adenosine (Sigma-Aldrich, Cat# A9251), guanosine (Sigma-Aldrich, Cat# G6752), cytidine (Sigma-Aldrich, Cat# C4654), and uridine (Sigma-Aldrich, Cat# U3750). ¹³C₅-Adenosine (Alsachim, Cat# C2291), ¹³C₅-guanosine (Alsachim, Cat# C2240), ¹³C₅-cytidine (Omicron, Cat# NUC-056), and ¹³C₅-uridine (Alsachim, Cat# M436) were used as internal standards. Analyst Software 1.6 (Sciex) was used for data acquisition and analysis. The intensity of measured nucleosides was normalized to internal standards and protein content of cell lysate pellets for intracellular nucleoside measurement and to intracellular RNA content for extracellular nucleoside measurement.

Hitzel J., Lee E., Zhang Y., Bibli S.I., Li X., Zukunft S., Pflüger B., Hu J., Schürmann C., Vasconez A.E., Oo J.A., Kratzer A., Kumar S., Rezende F., Josipovic I., Thomas D., Giral H., Schreiber Y., Geisslinger G., Fork C., Yang X., Sigala F., Romanoski C.E., Kroll J., Jo H., Landmesser U., Lusis A.J., Namgaladze D., Fleming I., Leisegang M.S., Zhu J, & Brandes R.P. (2018). Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells. Nature Communications, 9, 2292.

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Quantification of Prostaglandin E2 in Murine Hearts

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Directly after harvest, murine hearts were frozen in liquid nitrogen and mechanically homogenized without intermittent thawing. Concentrations of PGE₂ were determined using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) as described previously (Sisignano et al, 2016). In brief, PGE₂ was extracted from homogenized tissue samples (ca. 25 mg) using liquid–liquid extraction. PGE₂ was analyzed using a Synergi Hydro column (150 × 2 mm, 4 μm, Phenomenex) coupled to a hybrid triple quadrupole‐ion trap mass spectrometer QTRAP 5500 (Sciex, Darmstadt, Germany) equipped with a Turbo‐V‐source operating in negative ESI mode. The concentrations of the calibration standards, quality controls, and tissue samples were evaluated by Analyst software 1.6 and MultiQuant software 3.0 (Sciex) using the internal standard method (isotope dilution mass spectrometry). The calibration curve was calculated by quadratic regression with 1/x² weighting (calibration range 0.002–25 ng/sample).

Tóth A.D., Schell R., Lévay M., Vettel C., Theis P., Haslinger C., Alban F., Werhahn S., Frischbier L., Krebs‐Haupenthal J., Thomas D., Gröne H., Avkiran M., Katus H.A., Wieland T, & Backs J. (2018). Inflammation leads through PGE/EP 3 signaling to HDAC5/MEF2‐dependent transcription in cardiac myocytes. EMBO Molecular Medicine, 10(7), e8536.

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Toxin Profiling of G. polynesiensis

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In this study, two LC-MS/MS acquisition methods were used to determine the toxin profile of the strains of G. polynesiensis: method 1 for the P-CTX group based on the protocol by Sibat et al., 2018 [51 (link)] and method 2 for MTXs and gambierone group toxins [29 (link)]. A brief description is given below.
Both experiments were performed using a UHPLC system (UFLC Nexera, SHIMADZU, Kyoto, Japan) coupled to a hybrid triple quadrupole-linear ion-trap API4000 QTRAP mass spectrometer (SCIEX, Redwood city, CA, USA) equipped with a TurboV^® electrospray ionization source (ESI). The instrument control, data processing, and analysis were conducted using Analyst software 1.6.2 (Sciex, CA, USA).

Longo S., Sibat M., Viallon J., Darius H.T., Hess P, & Chinain M. (2019). Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia. Toxins, 11(12), 735.

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HPLC-MS/MS Analysis of Nicotine and Cotinine

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Sample preparations were analyzed via a HPLC-MS/MS system consisting of an Agilent 1100 (Santa Clara, CA) HPLC system coupled to an API-4000 mass spectrometer with TurboIonSpray source (SCIEX, Framingham, MA). The autosampler temperature was maintained at 10 °C. Chromatography was performed using a Phenomenex Luna Phenyl-Hexyl column (150 × 4.6 mm i.d., 3 µM particle size) and a Phenomenex SecurityGuard AQ C18 (4 × 2 mm) guard column. Five microliters of sample were injected, and the column was maintained at 30 °C. The flow rate was 0.8 mL/min, and the mobile phases consisted of 0.1 % formic acid in water with 5 mM ammonium formate (mobile phase A) and 0.1% formic acid in methanol (mobile phase B). The initial conditions were 20% B and held for 1 min. The linear gradient was increased to 95% B over 3 min and held at 95% B for 0.2 min before returning to initial conditions. MS detection was performed in positive ion mode with an ion source temperature of 500 °C, and an ion spray voltage of 2000 V. Transitions monitored were 163.20 → 84.0 for nicotine, 177.12 → 80.1 for cotinine, 166.10 → 89.1 for nicotine-d3, and 180.20 → 101.0 for cotinine-d3. Analyst software 1.6.2 (SCIEX, Redwood City, CA) was used for data acquisition and analysis.

Lefever T.W., Thomas B.F., Kovach A.L., Snyder R.W, & Wiley J.L. (2019). Route of Administration Effects on Nicotine Discrimination in Female and Male Mice. Drug and alcohol dependence, 204, 107504.

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UHPLC-MS/MS system for analysis

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The UHPLC-MS/MS system includes a Shimadzu Nexera X2 UHPLC system (Columbia, MD, USA) and a 4000 QTRAP^® MS/MS system (AB Sciex, Redwood City, CA, USA). System control and data analysis were performed using the Analyst^® software 1.6.2 (Sciex, Redwood City, CA, USA).

Gao X., Tsai R.Y., Ma J., Wang Y., Liu X., Liang D, & Xie H. (2021). Determination of Oxaliplatin by a UHPLC-MS/MS Method: Application to Pharmacokinetics and Tongue Tissue Distribution Studies in Rats. Pharmaceuticals, 15(1), 52.

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