The largest database of trusted experimental protocols

3 protocols using ape1 antibody

1

Molecular Targeting of STAT3 and EGFR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against p-STAT3 (Y705), STAT3, p-EGFR (Y1068), EGFR, BCL-xL, Survivin, α – Tubulin were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Anti-Stat3 (phospho Y705) antibody for immunohistochemistry (IHC) was purchased from Abcam (Cambridge, MA, USA). APE1 antibody was obtained from ThermoFisher Scientific (Waltham, MA, USA), c-MYC from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), Actin from Sigma-Aldrich (St. Louis, Missouri, USA) and p84 from Genetex (Irvine, CA, USA). The EGFR tyrosine kinase inhibitor, gefitinib, was purchased from LC laboratories (Woburn, MA). Transfection reagents (Polyjet and Lipojet) were purchased from SignaGen Laboratories (Rockville, MD). Control siRNA, EGFR siRNA and APE1 siRNA were obtained from Santa Cruz Biotechnology, Inc.
+ Open protocol
+ Expand
2

Antibody Sources and Inhibitors for NRF2 Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were purchased from commercial sources; NRF2 antibody from ABCAM (ab62352, Cambridge, MA, USA), APE1 antibody from Thermo Fisher Scientific (MA5-31586; Waltham, MA, USA), KEAP 1 antibody from Proteintech (10503-2-AP, Rosemont, IL, USA), β-actin antibody from Sigma-Aldrich (A5441), GSK-3-β (Total) (12456S), p-GSK-3-β (S9) (5558S), PARP, c-PARP and β-tubulin antibodies from Cell Signaling Technology (Danvers, MS, USA) and p84 from Genetex (Irvine, CA, USA). The E3330, an APE1 redox inhibitor, was obtained from Novus Biologicals (NBP1-49581, Centennial, CO, USA) and GSK-3β inhibitors; lithium chloride (Calbiochem) and CHIR-98014 (Selleck chemicals). Control siRNA and APE1siRNA were purchased from Dharmacon (ON-TARGET plus Human APEX1 siRNA, L-010237-00-0005). Transfection reagents, lipojet (for siRNAs) and polyjet (for plasmid DNAs), were purchased from SignaGen laboratories (Rockville, MD, USA).
+ Open protocol
+ Expand
3

Molecular Targeting of STAT3 and EGFR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against p-STAT3 (Y705), STAT3, p-EGFR (Y1068), EGFR, BCL-xL, Survivin, α – Tubulin were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Anti-Stat3 (phospho Y705) antibody for immunohistochemistry (IHC) was purchased from Abcam (Cambridge, MA, USA). APE1 antibody was obtained from ThermoFisher Scientific (Waltham, MA, USA), c-MYC from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), Actin from Sigma-Aldrich (St. Louis, Missouri, USA) and p84 from Genetex (Irvine, CA, USA). The EGFR tyrosine kinase inhibitor, gefitinib, was purchased from LC laboratories (Woburn, MA). Transfection reagents (Polyjet and Lipojet) were purchased from SignaGen Laboratories (Rockville, MD). Control siRNA, EGFR siRNA and APE1 siRNA were obtained from Santa Cruz Biotechnology, Inc.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!