The slices on slides were subjected to dewaxing and rehydration. Subsequently, permeabilization was performed using 1% Triton X-100 (#T8200; Solarbio) and blocking was performed with 10% horse serum. Next, the sections were incubated overnight at 4°C with the following primary antibodies: Ptgs2 (1:200, ab283574; Abcam, Cambridge, UK), Gpx4 (1:200, ab125066; Abcam), hepcidin (1:200, AF7003; Affinity, Liyang, China), IGF-1R (1:200, AF6125; Affinity), P-IGF-1R (1:200, AF3123; Affinity), P-AKT (1:200, AF0016; Affinity), ferritin (1:100, ab287968; Abcam) and TfR1 (1:2000, ab269513; Abcam). Then, the HRP-conjugated secondary antibody (PV-6000; ZSGB-BIO, Beijing, China) was used for incubation at 37°C for 20 min. Reaction products were visualized by using 3,3′-diaminobenzidine (DAB). The images were captured with the PA53 biological microscope.
Ab287968
Manufactured by Abcam
Sourced in China, United Kingdom
Ab287968 is a laboratory equipment product from Abcam. It is designed for general laboratory use. No further details about its core function or intended use are available.
Automatically generated - may contain errors
Lab products found in correlation
Ab269513, by Abcam (1 mentions)
Ab283574, by Abcam (1 mentions)
Ab125066, by Abcam (1 mentions)
Pv 6000, by ZSGB-BIO (1 mentions)
Af7003, by Affinity Biosciences (1 mentions)
Af0016, by Affinity Biosciences (1 mentions)
Af6125, by Affinity Biosciences (1 mentions)
T8200, by Solarbio (1 mentions)
Te2000 u fluorescence microscope, by Nikon (1 mentions)
Ab214039, by Abcam (1 mentions)
2 protocols using ab287968
1
Immunohistochemical Analysis of Oxidative Stress Markers
2
Quantifying Liver Iron and Transferrin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice liver sections were deparaffinized, rehydrated, and incubated with 3% H2O2 for 10 min. After blocking with 1% BSA for 1 h, the samples were incubated with anti-ferritin (abcam, ab287968, Cambridge, UK) or anti-transferrin (abcam, ab214039, Cambridge, UK) antibody overnight at 4 °C and then incubated with a fluorescein isothiocyanate-conjugated secondary antibody for 2 h. Immunoreactions were visualized under a TE2000-U fluorescence microscope (Nikon, Melville, NY, USA).
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