Total lysates prepared from subconfluent cells were immunoblotted with anti-MUC1-C (HM-1630-P1ABX, 1:400 dilution; ThermoFisher Scientific, Waltham, MA, USA), anti-PBRM1 (A301–591A, 1:10000; Bethyl Laboratories, Montgomery, TX, USA), anti-ARID2 (82342, 1:1000; Cell Signaling Technology (CST), Danvers, MA, USA), anti-BRD7 (15125, 1:1000; CST), anti-β-actin (A5441, 1:100,000; Sigma), anti-E2F1 (3742, 1:1000; CST), anti-SLC7A11/xCT (ab175186, 1:1000; abcam, Cambridge, MA, USA), anti-G6PD (8866, 1:1000; CST), anti-PGD (13389, 1:1000; CST), anti-BRG1 (ab110641, 1:10000; abcam, Cambridge, MA, USA), anti-ARID1A (12354, 1:500; CST), anti-OCT4 (2750, 1:1000 dilution; Cell Signaling Technology), anti-SOX2 (3579, 1:1000 dilution; Cell Signaling Technology), anti-KLF4 (12173, 1:1000 dilution; Cell Signaling Technology), anti-MYC (ab32072, 1:1000 dilution; Abcam, Cambridge, MA), anti-NANOG (4903, 1:1000; CST), anti-NOTCH1 (3608. 1:1000; CST), anti-BMI1 (6964, 1:1000, CST), anti-CD44 (KO601, 1:1000; TransGenic, Tokyo, Japan), anti-CD133 (5860, 1:1000; CST) and anti-GAPDH (5174, 1:5000, CST).
Anti pbrm1 a301 591a
Manufactured by Fortis Life Sciences
Sourced in United States
The Anti-PBRM1 (A301–591A) is a laboratory reagent that can be used to detect the presence of the PBRM1 protein in biological samples. PBRM1 is a subunit of the SWI/SNF chromatin remodeling complex and plays a role in gene regulation. The Anti-PBRM1 (A301–591A) can be used in various applications, such as western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of PBRM1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions)
Ab110641, by Abcam (1 mentions)
Ab175186, by Abcam (1 mentions)
Anti oct4, by Cell Signaling Technology (1 mentions)
Anti muc1 c hm 1630 p1abx, by Thermo Fisher Scientific (1 mentions)
Ab32072, by Abcam (1 mentions)
Ab109617, by Abcam (1 mentions)
Ab180589, by Abcam (1 mentions)
Anti fbxw7, by Abcam (1 mentions)
Anti wars, by GeneTex (1 mentions)
Anti muc1 c, by Cell Signaling Technology (1 mentions)
Anti isg15, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti pbrm1 a301 591a
1
Immunoblotting Analysis of Stem Cell Markers
2
Immunoblotting Analysis of Cell Signaling
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Whole cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA). Immunoblotting was performed with anti-MUC1-C (16564, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA, USA), anti-STAT1 (9172S, 1:1000 dilution; CST), anti-IRF1 (#8478S, 1:1000 dilution; CST), anti-IFNGR1 (34808, 1:1000; CST), anti-GAPDH (#5174S, 1:1000 dilution; CST) and anti-β-actin (A5441; 1:100000 dilution; Sigma, St. Louis, MO, USA), anti-FBXW7 (ab109617, 1:1000; abcam, Cambridge, MA, USA), anti-MTA1 (5647, 1:1000; CST), anti-MBD3 (14540, 1:1000; CST), anti-IDO1 (86630, 1:1000; CST), anti-WARS (GTX110223, 40037, 1:1000; Gene Tex), anti-PTGES (ab180589, 1:1000; abcam), anti-PBRM1(A301-591A, 1:10000; Bethyl Laboratories, Montgomery, TX, USA), anti-ARID1A (12354, 1:500; CST), anti-ISG15 (sc166755, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-SERPINB9 (PA5-51038, 1:2000; Invitrogen, Waltham, MA, USA).
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