Nrf 2 bs 1074r

(R)-sal, (S)-sal, and (RS)-sal were supplied by Dongguan Key-Pharma Biomedical Company (Guangdong, China). DSS (MW 36-50 kDa) was obtained from MP Biomedicals Co., Ltd. (California, USA). The positive drug 5-ASA was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The reagent for detecting fecal occult blood was supplied by Baso Diagnostics, Inc. (Zhuhai, China). The enzyme-linked immunosorbent assay (ELISA) kits for detecting the inflammatory cytokines interferon-γ (IFN-γ), interleukin- (IL-) 1β, IL-6, and tumor necrosis factor-α (TNF-α) were offered by Neobioscience Technology Company (Shenzhen, China). The antibodies of β-actin (ab227387), occludin (ab216327), and CD4 (ab183685) were obtained from Abcam Inc. (Burlingame, USA). The antibodies of zonula occludens-1 (ZO-1) (bs-1329R) and Nrf-2 (bs-1074R) were obtained from Bioss (Beijing, China). The antibodies of NF-κB p65 (10745-1-AP) and heme oxygenase-1 (HO-1) (10701-1-AP) were purchased from Proteintech (Rosemont, USA). The antibody of phospho-NF-κB p65 (p-NF-κB p65) was purchased from Cell Signaling Technology (Danvers, USA). The antibodies of transforming growth factor-β1 (TGF-β1) (BA0290) and alpha smooth muscle actin (α-SMA) (BM0002) were offered by Boster Biological Technology Co., Ltd. (Wuhan, China). All chemical reagents employed were of analytical grade.

Antibodies against iNOS (ab3523), Cox-2 (ab179800), HO-1 (ab13243), and IκB-α (ab32518) were purchased from Abcam (Cambridge, UK). NF-κB p65 (sc-372) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phosphorylated (p)-IKKα/β (#2697) was purchased from Cell Signaling Technology (Danvers, MA, USA). Nrf2 (bs-1074R) was purchased from Bioss Antibodies (Woburn, MA, USA). 5-ALA was provided by Neopharma Japan (Tokyo, Japan). SFC was obtained from Komatsuya Corporation (Osaka, Japan). LPS from Salmonella typhimurium and Pred were purchased from Sigma-Aldrich (St. Louis, MO, USA).

To acquire the requisite proteins for our research, we utilize a modified RIPA buffer to lyse the tissue, as previously outlined in our study [9 (link)]. The primary antibodies of Sirt3(ab217319, 1:1000), TfR1 (ab214039, 1:1000), DMT1 (ab55735, 1:1000), FTH1 (ab183781, 1:1000), xCT (ab175186, 1:1000), and FSP1(ab197896, 1:1000) were purchased from Abcam (Cambridge, MA, USA), the primary antibodies of IRP1 (bs-9848R, 1:1000), FPN1 (bs-21360R, 1:1000), GPX4 (bs-3884R, 1:5000), Nrf2 (bs-1074R, 1:1000), p-Nrf2 (bs-23531R, 1:1000), and SOD2 (bs-1080R, 1:1000) were purchased from Bioss (Beijing, China), and the primary antibodies of β-actin (66009-1-Ig, 1:2000) were purchased from Proteintech (Chicago, IL, USA). In this study, the secondary antibodies used are HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (Cat No. SA00001-1, 1:5000) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (Cat No. SA00001-2, 1:5000) were purchased from proteintech (Chicago, IL, USA). ECL Western blotting substrate was utilized for the development of blots, while the UVP Auto Chemi Image system (Tanon 4600SF, Shanghai, China) was employed to visualize luminescence.