Ppara

Manufactured by Proteintech

PPARA is a protein that functions as a nuclear receptor. It regulates the expression of genes involved in lipid and carbohydrate metabolism.

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Lab products found in correlation

2 protocols using ppara

Western Blot Analysis of Ferroptosis Regulators

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Cells were lysed with RIPA (R0020, Solarbio, Beijing) containing Protease and Phosphatase Inhibitor Cocktail (P002, NCM Biotech, Suzhou) and collected for centrifugation. The steps in this laboratory are described in the previous article about Western experiments. Antibodies used are as follows: FAM60A (1:1,000, Novus, H00058516-B02P), GPX4 (1:1,000, Proteintech, 67763), ACSL1 (1:1,000, Proteintech, 13989), ACSL4 (1:1,000, Proteintech, 22401), PPARA (1:1,000, Proteintech, 15540), PPARG (1:1,000, Proteintech, 16643), YY1 (1:1,000, CST, #46395), MST1 (1:1,000, CST, #14946), p-MST1/2 (1:800, Abcam, ab79199), YAP (1:1,000, CST, #14074), p-YAP (1:700, CST, #13008), p-LATS1 (1:1,000, CST, #9157), LATS1/2 (1:1,000, Bethyl Laboratories, Montgomery, TX), and ACTB (1:1,000, Abways, AB0035).

Pan H., Sun Y., Qian L.H., Liao Y.N., Gai Y.Z., Huo Y.M., Li Z.Q, & Nie H.Z. (2024). A Nutrient-Deficient Microenvironment Facilitates Ferroptosis Resistance via the FAM60A–PPAR Axis in Pancreatic Ductal Adenocarcinoma. Research, 7, 0300.

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Metabolic Regulation in Cell Culture

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Recombinant TGF-β1 and recombinant PDGF-BB were purchased from PEPROTECH. α-ketoglutarate (α-KG), IR-780, dimethyl malonate (DMM), dimethyl succinate and aminooxyacetate (AOA) were obtained from Sigma-Aldrich. Fenofibrate, sodium succinate, glucose and oligomycin were purchased from Selleck. 2-DG, BAY876, 3-PO, etomoxir, bleomycin, AICAR and vigabatrin were obtained from MCE. Palmitate-conjugated BSA was purchased from Seahorse Bioscience. Oil Red O was from Nanjing Jiancheng Bio-engineering.
Antibodies to GLUT1 (AB_10837075), GLUT3 (AB_10694437), PFK1 (AB_10996606), PPARA (AB_2252506), PPARG (AB_10596794), PGC1A (AB_2828002), PGC1B (AB_2879093), ACOX1 (AB_2221670), CPT1A (AB_2084676), α-SMA (AB_2223009), fibronectin (AB_2105691), Collagen I (AB_2082037), β-Actin (AB_2687938), SDHA (AB_11182164), SDHB (AB_2285522), SDHC (AB_2183291), SLCO4A1 (AB_2880501), HK1 (AB_2878162), PFK2 (AB_2162854), SDHD (AB_2880540), anti-CD36 (AB_10597244) were purchased from Proteintech. Antibodies to HIF-1α (AB_296474), HK2 (AB_2868547) were obtained from Abcam (The number inside the brackets is the Research Resource Identifier (RRID) of antibody). Secondary antibodies to different species IgG of Alexa Fluor® 594 (red) or 488 (green) were purchased from Invitrogen. DAPI and Hoechst 33342 were obtained from Beyotime.

Wang Z., Chen L., Huang Y., Luo M., Wang H., Jiang Z., Zheng J., Yang Z., Chen Z., Zhang C., Long L., Wang Y., Li X., Liao F., Gan Y., Luo P., Liu Y., Wang Y., X.u.T.a.n., Zhou Z., Zhang A, & Shi C. (2021). Pharmaceutical targeting of succinate dehydrogenase in fibroblasts controls bleomycin-induced lung fibrosis. Redox Biology, 46, 102082.

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