Cea907ge 96t

The platelets were extracted with the human peripheral‐blood platelet separation solution (Batch No.: PLA2014TBD; Tianjin Haoyang Biological Manufacture Co., Ltd., China). The centrifugal extraction of platelets and plasma was performed with low‐speed table‐top centrifuges (Model No.: TDL‐50B; Shanghai Anting Scientific Instrument Factory, China). The extracted platelets and plasma were placed in a −80°C refrigerator for a period of no more than 2 months. The levels of 5‐HT in the plasma and platelets were detected with an enzyme‐linked immunosorbent assay (ELISA) kit for calcitonin gene–related peptide (Cloud‐Clone Corp., Wuhan, China, No.: CEA876Hu 96T).
²⁹ (link) The levels of NE and DA in the plasma were respectively measured with an ELISA kit for noradrenaline (Cloud‐Clone Corp., No.: CEA907Ge 96T) and one for dopamine (Cloud‐Clone Corp., No.: CEA851Ge 96T).
³⁰ (link)

After the behavioral measurement of the corresponding cold exposure, the mice were euthanized to collect blood samples. Immediately, the serums were isolated and stored at –80 °C until analysis. The levels of serum Cort were determined by the commercial ELISA kit (#CEA540Ge 96T, Cloud-Clone Corp, Katy, TX, USA). The levels of serum CRH were determined by the commercial ELISA kit (#CEA835Mu 96T, Cloud-Clone Corp, Katy, TX, USA). The levels of serum E were determined by the commercial ELISA kit (#CEA858Ge 96T, Cloud-Clone Corp, Katy, TX, USA). The levels of serum NE were determined by the commercial ELISA kit (#CEA907Ge 96T, Cloud-Clone Corp, Katy, TX, USA). The levels of serum 5-HT were determined by the commercial ELISA kit (#CEA808G 96T, Cloud-Clone Corp, Katy, TX, USA). The levels of serum DA were determined by the commercial ELISA kit (#CEA851Ge 96T, Cloud-Clone Corp, Katy, TX, USA). All operations were carried out in strict accordance with the instructions of the kit.