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L citrulline

Manufactured by Cambridge Isotopes
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L-citrulline is a non-essential amino acid produced by the body. It is an important intermediate in the urea cycle, which helps remove waste nitrogen from the body. L-citrulline is available as a laboratory product for research and analytical purposes.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Bovine brain calmodulin, by Merck Group (1 mentions) L ng nitroarginine methyl ester l name, by Merck Group (1 mentions) Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) 15n4 arg, by Cambridge Isotopes (1 mentions) 15n nitrite, by Cambridge Isotopes (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (1 mentions) Optima lc ms grade, by Thermo Fisher Scientific (1 mentions) Acetyl d3 carnitine, by Cambridge Isotopes (1 mentions) Hypoxanthine, by Merck Group (1 mentions) L proline, by Cambridge Isotopes (1 mentions) Hplc grade formic acid, by Thermo Fisher Scientific (1 mentions) L arginine, by Cambridge Isotopes (1 mentions) Hplc grade ammonium acetate, by Merck Group (1 mentions) L methionine, by Cambridge Isotopes (1 mentions) 2h9 isovalerylcarnitine, by Cambridge Isotopes (1 mentions) 2h3 octanoylcarnitine, by Cambridge Isotopes (1 mentions) 2h9 myristoylcarnitine c14, by Cambridge Isotopes (1 mentions)

3 protocols using l citrulline

1

Nitric Oxide Synthesis Assay Protocol

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ARG [as L-arginine HCl], CIT [as L-citrulline], 4-(2-Hydroxyethyl)-1 piperazineethanesulfonic acid (HEPES), CaCl2·4H2O, bovine brain calmodulin, (6R)-5,6,7,8-tetrahydro-l-biopterin (BH4), NADPH, ethylene diamine tetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), and L-NG-Nitroarginine methyl ester (L-NAME) were purchased from Sigma (St. Louis, MO). 15N4-ARG [as its HCl, (U-15N4, 98%)], the internal standard for ARG, 13C6-ARG [as ARG:HCl (U-13C6, 98%)], the internal standard for CIT, D4-CIT [as L-citrulline (4,4,5,5-D4, 96.5 %)], and 15N-nitrite [as NaNO2 (15N, 98%+)] were obtained from Cambridge Isotope Laboratories, Inc. These compounds were used without further purification. Cell culture reagents were purchased from Invitrogen.
Shin B.S., Fung H.L., Upadhyay M, & Shin S. (2015). Estimation of nitric oxide synthase activity via LC-MS/MS determination of 15N3-citrulline in biological samples. Rapid communications in mass spectrometry : RCM, 29(5), 447-455.
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2

Targeted Metabolomics Analysis Protocol

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All reagents were LC–MS optima grade (Fisher Scientific, Hanover Park, IL, USA) and all standards were of the highest purity available. Standards were acquired from Sigma-Aldrich (St. Louis, MO, USA) (hypoxanthine, L-carnitine, acetyl-L-carnitine, propionyl-L-carnitine, isobutyryl-L-carnitine, 2-methylbutyryl-L-carnitine, 7-methylguanine, creatine, xanthine, asymmetric dimethylarginine [ADMA], guanine, oleamide, L-citrulline, L-proline, taurine, 4-nitrobenzoic acid, and debrisoquine sulfate), CDN isotopes (Pointe-Claire, QC, Canada) (carnitine-d3, acetyl-d3-carnitine, citrulline-d7, and proline-d3), and Cambridge Isotope Laboratories, Inc. (Andover, MA, USA) (hypoxanthine-d3 and taurine-13C2).
Pannkuk E.L., Laiakis E.C., Gill K., Jain S.K., Mehta K.Y., Nishita D., Bujold K., Bakke J., Gahagen J., Authier S., Chang P, & Fornace AJ J.r. (2019). Liquid Chromatography–Mass Spectrometry-Based Metabolomics of Nonhuman Primates after 4 Gy Total Body Radiation Exposure: Global Effects and Targeted Panels. Journal of proteome research, 18(5), 2260-2269.
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3

Comprehensive Lipid and Metabolite Analysis

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PC(12:0/13:0), PE(12:0/13:0), SM(d18:1/12:0) and Cer(d18:1/12:0) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Stable isotope labeled references were purchased from Cambridge Isotope Laboratories (Andover, MA, USA), including: Glycine (2-13C, 15N), L-Alanine (2,3,3,3-D4), L-Valine (D8), L-Leucine (5,5,5-D3), L-Methionine (methyl-D3), L-Phenylalanine (ring-13C6), L-Tyrosine (ring-13C6), L-Aspartic acid (2,3,3-D3), DL-Glutamic acid (2,4,4-D3), L-Ornithine: HCl (5,5-D2), L-Citrulline (5,5-D2) and L-Arginine (5-13C, 4,4,5,5-D4). Deuterium-labeled carnitine and acylcarnitines (NSK-B set) were purchased from Cambridge Isotope Laboratories (Tewksbury, MA, USA), including 2H3-Acetylcarnitine (C2), 2H3-Propionylcarnitine (C3), 2H3-Butyrylcarnitine (C4), 2H9-Isovalerylcarnitine (C5), 2H3-Octanoylcarnitine (C8), 2H9-Myristoylcarnitine (C14), and 2H3-Palmitoylcarnitine (C16). HPLC-grade ammonium acetate was purchased from Sigma-Aldrich (St. Louis, MO, USA), and HPLC-grade formic acid and acetonitrile were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All solutions were prepared by LC-MS ultra-pure water.
Zhao H., Xi C., Tian M., Lu X., Cai T.J., Li S., Tian X.L., Gao L., Liu H.X., Liu K.H, & Liu Q.J. (2020). Identification of Potential Radiation Responsive Metabolic Biomarkers in Plasma of Rats Exposed to Different Doses of Cobalt-60 Gamma Rays. Dose-Response, 18(4), 1559325820979570.
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