Secondary goat anti mouse 554002

Rapamycin was purchased from MedChemExpress LLC (NJ 08540, USA). MTT, protease and phosphatase inhibitors were from Sigma-Aldrich (Oakville, Ontario, Canada). LDH-Cytotoxicity Colorimetric Assay Kit II was purchased from BioVision (Milpitas, California, USA), while Annexin V-FITC/PI Kit was from BD Bioscience. (Mississauga, ON, Canada). Autophagy Assay, Red (Cat. #9156), Intracellular Total ROS Activity Assay (Cat. #9144) and Intracellular GSH Assay (Cat. #9137) were purchased from ImmunoChemistry Technologies (Davis, CA, USA). Autophagy Inhibitor, 3-MA (Cat. #189490) and N-acetylcysteine (NAC) were from Sigma. ECL system was acquired from EMD Millipore (Billerica, MA, USA). The primary antibodies as procaspase 3 (sc-56046), procaspase 9 (sc-17784), NF-κB (sc-8008) and β-catenin (sc-59737) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), E-cadherin (8834), pERK1/2 (4370), ERK1/2 (4695), pp38 (4631), p38 (9212), cleaved caspase-3 (9664S), cleaved caspase-9 (20750S) were from Cell Signaling Technology (Danvers, MA, USA), LC3B (2775) and p62 (39,749) were all from Cell Signaling Technology (Danvers, MA, USA)and β-actin (A5441) was from Sigma-Aldrich (Oakville, ON, Canada). The secondary goat anti-mouse (554002) and anti-rabbit (554021) were from BD Pharmingen (Mississauga, ON, Canada). VersaDoc™ MP 5000 system was from Bio-Rad (Mississauga, ON, Canada).

Western blot was used as described in our previous work [24 (link)]. The antibodies used are as follows: NF-κB (sc-8008) and β-Catenin (sc-59737) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); pERK1/2 (4370) and ERK1/2 (4695) were purchased from Cell Signaling Technology (Danvers, MA, USA), and GADPH was purchased from Sigma Aldrich. The secondary goat anti-mouse (554002) and anti-rabbit (554021) were from BD Pharmingen (Mississauga, ON, Canada). Each experiment was repeated three times.