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SNU387 is a laboratory equipment designed for the analysis and detection of biomolecules. It utilizes spectrophotometric techniques to quantify the concentration and purity of various biological samples, such as proteins, nucleic acids, and other macromolecules. The core function of SNU387 is to provide accurate and reliable measurements to support scientific research and development activities.

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2 protocols using snu387

1

Culturing and Authentication of HCC Cell Lines

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HCC cell lines, SKHEP1 (RRID: CVCL_0525), HepG2 (RRID: CVCL_0027), SNU387 ((RRID: CVCL_0250), and Huh7 (RRID: CVCL_0336) were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan), while the cell lines Hep3B (RRID: CVCL_0326), HA22T (RRID: CVCL_7046), and PLC5 (RRID: CVCL_0485) were obtained from American Type Culture Collection (Manassas, VA, USA). All the cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Grand Island, NY, USA), which was supplemented with 10% Fetal Bovine Serum (FBS) (Thermo Fisher Scientific), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Thermo Fisher Scientific). The culturing of the cells was performed in a humidified tissue culture incubator with a 5% CO2 atmosphere at 37 °C. All human cell lines have been authenticated using short tandem repeat profiling within three years. All experiments were performed with mycoplasma-free cells.
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2

Culturing and Authentication of HCC Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCC cell lines, SKHEP1 (RRID: CVCL_0525), HepG2 (RRID: CVCL_0027), SNU387 ((RRID: CVCL_0250), and Huh7 (RRID: CVCL_0336) were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan), while the cell lines Hep3B (RRID: CVCL_0326), HA22T (RRID: CVCL_7046), and PLC5 (RRID: CVCL_0485) were obtained from American Type Culture Collection (Manassas, VA, USA). All the cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Grand Island, NY, USA), which was supplemented with 10% Fetal Bovine Serum (FBS) (Thermo Fisher Scientific), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Thermo Fisher Scientific). The culturing of the cells was performed in a humidified tissue culture incubator with a 5% CO2 atmosphere at 37 °C. All human cell lines have been authenticated using short tandem repeat profiling within three years. All experiments were performed with mycoplasma-free cells.
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