Cells were grown and treated with 10 μM MC or DMC on coverslips. Untreated and treated cells were fixed with 4% Formaldehyde and permeabilized with 0.5% Triton-X-100. Immunohistochemistry was done with ATR (Abcam, ab2905) and Lamin A/C (Millipore, MAB3211) antibodies followed by incubation with FITC-conjugated anti-Rabbit (Invitrgen A11037) and Alexa-conjugated anti-mouse (Invitrogen A11058). Coverslips were mounted onto slides using Vectashield mounting medium with DAPI (Fisher Scientific NC9524612, Pittsburgh, PA, USA). Images were collected by PerkinElmer UltraVIEW ERS Spinning Disc Microscope, Waltham, MA, USA. All images were captured using a 100× objective.
Ultraview ers spinning disc microscope
Manufactured by PerkinElmer
Sourced in United States
The UltraVIEW ERS Spinning Disc Microscope is a high-speed confocal imaging system that provides real-time acquisition of high-quality images. It utilizes a spinning disc technology to enable fast, sensitive, and versatile live-cell imaging.
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Lab products found in correlation
2 protocols using ultraview ers spinning disc microscope
1
Immunodetection of ATR and Lamin A/C
2
Time-lapse Imaging of Centrin-GFP MDA-MB-231 Cells
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Stably transfected centrin-GFP MDA-MB-231 cells were plated at 60% confluence on Matek cell culture plates. Cells were then imaged using time-lapse microscopy at 40X magnification on PerkinElmer Ultra View ERS spinning disc microscope (Waltham, MA, USA). GFP images were captured at multiple points every 10 min for 18 h. Captured images from each experiment were analyzed using Volocity software (Improvision Coverty, UK).
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