Isotype control human igg

CP-724714 HER2 inhibitor was purchased from Selleckchem (Houston, TX, USA). Lapatinib and isotype control human IgG were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trastuzumab was purchased from Roche (Basel, Switzerland). Mouse monoclonal anti-human HER2 (9G6) and (A-2), anti-human EGFR (A-10), and anti-human HER3 (RTJ.2) antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rabbit polyclonal anti-human phospho-HER2 Y-1005, Y-1112, Y-1127, Y-1139, Y-1196, and Y-1248 antibodies were purchased from FroggaBio (Toronto, ON, Canada). All other chemicals were purchased from Sigma-Aldrich.

HER2 kinase inhibitor CP-724714 was purchased from Selleckchem (Houston, TX, USA). Pertuzumab (Perjeta^®) and trastuzumab (Hercepton^®) were purchased from Hoffmann-La Roche (Basel, Switzerland). Mouse monoclonal anti-human HER2 (9G6) (sc-08), anti-human HER2 (A-2) (sc-393712), anti-human EGFR (A-10) (sc-373746), anti-human HER3 (RTJ.2) (sc-415), and rabbit polyclonal anti-human pY1248 HER2 (sc-12352-R) antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rabbit polyclonal anti-human pY1005, pY1112, pY1127, pY1139, and pY1196 HER2 were purchased from FroggaBio (Toronto, ON, Canada) and mouse monoclonal anti-human pY1221/1222 HER2 (6B12) (2243) were from Cell Signaling Technology (Danvers, MA, USA). Anti-rabbit and anti-mouse RDye^® 800CW and RDye^® 650 secondary antibodies were purchased from LI-COR biotechnology Inc. (Lincoln, NE, USA). Isotype control human IgG and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).