Mouse il 1β elisa kit

Manufactured by Dakewe

Sourced in China

The Mouse IL-1β ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of mouse interleukin-1β levels in cell culture supernatants, serum, and plasma samples.

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Lab products found in correlation

Prostaglandin e2 express elisa kit, by Cayman Chemical (1 mentions) Legend max mouse tnf α elisa kit, by BioLegend (1 mentions) Mouse il 18 elisa kit, by Abcam (1 mentions) Anti mmp11, by Thermo Fisher Scientific (1 mentions) Anti tsg101, by Abcam (1 mentions) Albumin from chicken egg white, by Merck Group (1 mentions) Cd40 pe, by BioLegend (1 mentions) Tmb elisa substrate, by Abcam (1 mentions) Quant ittm picogreendsdna reagent, by Thermo Fisher Scientific (1 mentions) Mouse il 12p70 elisa kit, by Dakewe (1 mentions) Phenol red, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Lps l2880, by Merck Group (1 mentions) Atp tlrl atpl, by InvivoGen (1 mentions) Msu tlrl msu, by InvivoGen (1 mentions) Human il 1β elisa kit, by Dakewe (1 mentions) Perfectstart green qpcr supermix, by Transgene (1 mentions) Nucleozol, by Macherey-Nagel (1 mentions) Mouse il 6 elisa kit, by Dakewe (1 mentions) Mouse tnf α elisa kit, by Dakewe (1 mentions)

5 protocols using mouse il 1β elisa kit

Quantification of Inflammatory Markers in BV2 Cells

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The levels of TNF-α (LEGEND MAX™ Mouse TNF-α ELISA Kit, Biolegend), IL-1β (Mouse IL-1β Elisa kit, Dakewe Biotech Co. 1210122) and COX2 (Prostaglandin E2 Express ELISA Kit, Cayman #500141) in the supernatant of BV2 cell culture adding LPS with or without mNPC-exos were quantified using ELISA kits. The kits were used as described previously [14 (link)].

Bian B., Zhao C., He X., Gong Y., Ren C., Ge L., Zeng Y., Li Q., Chen M., Weng C., He J., Fang Y., Xu H, & Yin Z.Q. (2020). Exosomes derived from neural progenitor cells preserve photoreceptors during retinal degeneration by inactivating microglia. Journal of Extracellular Vesicles, 9(1), 1748931.

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Mouse Cytokine Quantification ELISA

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Serum IL-1β and IL-18 levels were measured using commercially available ELISA kits according to their respective manufacturer’s instructions (Mouse IL-18 ELISA Kit, ab216165, Abcam; Mouse IL-1β ELISA kit, DKW12-2012-096, Dakewe Biotech, China).

Ni H., Ou Z., Wang Y., Liu Y., Sun K., Zhang J., Zhang J., Deng W., Zeng W., Xia R., Xu J., Gong N, & Miao Y. (2023). XBP1 modulates endoplasmic reticulum and mitochondria crosstalk via regulating NLRP3 in renal ischemia/reperfusion injury. Cell Death Discovery, 9, 69.

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Western Blotting Antibodies and IL-1β Measurement

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Western blotting was performed as previously reported.⁵¹ (link) Antibodies used in the experiments were anti-CD9 (CST, #13403), anti-TSG101 (Abcam, #ab125011), anti-ZEB1 (CST, #3396), anti-HIF1α (CST, #36169), anti-E-cadherin (CST, #3195), anti-vimentin (CST, #5741), anti-GLUT4 (CST, #2213), anti-PPARγ (CST, #2435), anti-HSL (CST, #18381), anti-α-SMA (CST, #19245), anti-MMP11 (Invitrogen, #SN74-08), anti-collagen I (CST, #72026), and anti-GAPDH (CST, #5174). The mouse IL-1β in culture medium was measured in the coculture or non-coculture experimental conditions using a Mouse IL-1β ELISA Kit (Dakewe Biotech, #2012096).

Sun Z., Sun D., Feng Y., Zhang B., Sun P., Zhou B., Du L., Wang Y., Fan Z., Yang J., Li Y., Hu S, & Zhan H. (2021). Exosomal linc-ROR mediates crosstalk between cancer cells and adipocytes to promote tumor growth in pancreatic cancer. Molecular Therapy. Nucleic Acids, 26, 253-268.

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Murine Dendritic Cell Activation Assay

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DMEM (C11995500BT), Fetal bovine serum qualified Australia (10099141C) and Trypsin-EDTA (0.25%), phenol red (25200072) were purchased from Gibco; CD11c-FITC (117306), CD86-PerCP (105026), CD80-APC (104714) and CD40-PE (124610) were purchased from Biolegend; Goat Anti-Mouse IgG Antibody (HRP) (ab98762), Goat Anti-Mouse IgA alpha chain (HRP) (ab97235), Goat Anti-Mouse IgG1 heavychain (HRP) (ab97240), Goat Anti-Mouse IgG2a heavy chain (HRP) (ab97245), TMB ELISA Substrate (ab171523) and 450nm stop solution for TMB substrate (ab171529) were purchased from Abcam; Penicillin-Streptomycin Solution100X (C0222), and BSA, FranctionV (ST023) were purchased from Beyotime; Mouse IFN-gamma ELISpot PLUS kit (HRP) (3321-4HPW-2), Mouse TNFa ELISA kit (1217202), Mouse IL-1β ELISA kit (1210122), and Mouse IL-12p70 ELISA kit (1211202) were purchased from Dakewe; GM-CSF (315-02-10ug) and IL-4 (214-14-20ug) were purchased from PEPROTECH; Quant-iTTM PicoGreendsDNA Reagent (P7581) was purchased from Thermo Scientific; 2019-nCoV Protein RBD (DRA36) was purchased from novoprotein; Albumin from chicken egg white (A5503) was purchased from sigma.

Mao L., Liu C., Liu J.Y., Jin Z.L., Jin Z., Xue R.Y., Feng R., Li G.C., Deng Y., Cheng H., Zou Q.M, & Li H.B. (2022). Novel Synthetic Lipopeptides as Potential Mucosal Adjuvants Enhanced SARS-CoV-2 rRBD-Induced Immune Response. Frontiers in Immunology, 13, 833418.

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Inflammatory Cytokine Assay Protocol

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Dulbecco’s modified Eagle medium (DMEM, high glucose, 11965092), fetal bovine serum (FBS, 10099141), Lipofectamine™ 2000 (11668019), Pierce™ BCA Protein Assay Kit (23225), and Thermo Scientific RevertAid Mix (M16325) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Protease inhibitor cOmplete™ Cocktail (4693116001) was purchased from Roche (Basel, Switzerland). PerfectStart™ Green qPCR SuperMix (AQ601) was purchased from TransGen Biotech (Beijing, China). NucleoZOL (740404) was purchased from MACHEREY-NAGEL (MN, Düren, Deutschland). Mouse IL-1β ELISA kit (1210122), human IL-1β ELISA kit (1110122), mouse IL-6 ELISA kit (1210602), and mouse TNF-α ELISA kit (1217202) were purchased from DAKEWE (Shenzhen, China). LPS (L2880) was purchased from Sigma (Missouri, USA). ATP (tlrl-atpl) and MSU (tlrl-msu) were purchased from In vivoGen.

Li J., Lin H., Fan T., Huang L., Zhang X., Tai Y., Fang Y., Li Q., Zhao R., Wang P., Zhou L., Wan L., Wu Y., Zhong H., Wei C, & Yang X. (2023). BPOZ-2 is a negative regulator of the NLPR3 inflammasome contributing to SARS-CoV-2-induced hyperinflammation. Frontiers in Cellular and Infection Microbiology, 13, 1134511.

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