Blood samples were assembled from all individuals after clinical determination and blood samples were inspected for routine laboratory examinations, including biochemical profile [liver function tests] and [kidney function tests] using an automated biochemistry analyzer (Beckman Coulter AU680 chemistry analyzer, USA). Complete blood count was estimated using an automated hematology analyzer (Sysmex XN 1000, Japan). Serum CA19.9 and CEA were estimated by ELISA assay according to the manufacturer’s protocol (ARCHITECT i 1000 SR immunoassay analyzer, Abbott USA). LRG1 (Assay Pro/Human LRG1, MO, USA) and SCF Glory Science Co. Ltd/Human SCF/Del Rio, TX, USA) were determined by immunoassay according to the manufacturer’s protocol.
Architect i1000sr immunoassay analyzer
Manufactured by Abbott
Sourced in United States
The ARCHITECT i1000SR is an automated immunoassay analyzer designed for in vitro diagnostic testing. It is capable of performing a variety of immunoassay tests, including those for hormones, cardiac markers, infectious diseases, and other analytes. The system is designed for high-throughput laboratory settings, offering efficient processing and reliable results.
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8 protocols using architect i1000sr immunoassay analyzer
1
Comprehensive Biomarker Evaluation in Clinical Samples
2
Comprehensive Metabolic Profiling of Hemodialysis Patients
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Serum samples were collected at baseline, prior to HD, from all patients at the start of the randomized controlled trial after the 2-week washout phase. Subsequently, samples were collected 2, 4, and 6 weeks after the start of medication, and, thereafter, monthly blood tests were conducted according to the hospital protocol. We compared the levels of hemoglobin (Hb), fasting plasma glucose, HbA1c, P, corrected Ca, magnesium (Mg), intact parathyroid hormone (int-PTH), albumin, total cholesterol, high-density lipoprotein cholesterol, triglycerides, C-reactive protein (CRP), osteocalcin (OC), and bone alkaline phosphatase (BAP), at the various time points. Blood was analyzed using a JCA-BM6050™ auto-analyzer (JEOL, Tokyo, Japan). The total serum Ca level was adjusted according to albumin level as follows: corrected Ca = Ca + 0.8 × (4.4 – albumin) g/dL. Int-PTH levels were measured using an immunometric assay (ARCHITECT i1000SR Immunoassay Analyzer; Abbott Laboratories, Abbott Park, IL, USA), as were OC levels (Mitsubishi Yuka BGP IRMA kit; LSI Medience Corporation, Tokyo, Japan) and BAP levels (Access Ostase®; Beckman Coulter, Brea, CA, USA).
3
Serum PSA Measurement Protocol
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Blood samples were collected in the morning using vacuum blood collection tubes with 10 mL plastic clot activator with a diameter of 16 × 100 mm, and the elderly were instructed to remain fasting for eight hours, not to have sexual intercourse and to avoid horse displacement, motorcycle and bicycle use for 72 h before collection. After venipuncture, the blood was stored, to be subsequently centrifuged (procedure carried out in a balanced way, checking the calibration and matching the tubes according to their physical characteristics) in pairs for 10 min at 3000 rpm, using an Inbrás ALB 18 VT Serological centrifuge (Inbrás, Ribeirão Preto, BRAZ/SP, Brazil), then the serum was transferred with the aid of an automatic pipette and variable volume to a tube properly labeled with a minimum of 2.0 mL of serum, and stored in an ice box and then transferred to the laboratory that performed the sample processing, following the Standard Operating Procedure (SOP) [23 ] and ARCHITECT i1000SR immunoassay analyzer (Abbott, Green Oaks, USA/IL, United States of America). A PSA level equal to or greater than 4 ng/mL was considered high [13 (link),15 (link),24 (link)].
4
Quantification of SARS-CoV-2 Antibody Titers
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The titers of IgG antibodies to SARS-CoV-2 spike RBD were determined using a chemiluminescent microparticle immunoassay (SARS-CoV-2 IgG II Quant Assay; Abbott Laboratories, IL, USA) according to the manufacturer’s instructions, by ARCHITECT i1000SR immunoassay analyzer (Abbott). The results are expressed in arbitrary units/mL (AU/mL). The seropositivity was defined for titers ≥ 50 AU/mL.
According to the WHO standard preparation for SARS-CoV-2 binding antibodies (11 (link), 12 (link)), a conversion factor from Abbott AU became available (1 BAU/mL = 0.142 × AU/mL), and the results of the present study have been expressed in BAU/mL. The cut-off of 7.1 BAU/mL was used to define seropositivity.
According to the WHO standard preparation for SARS-CoV-2 binding antibodies (11 (link), 12 (link)), a conversion factor from Abbott AU became available (1 BAU/mL = 0.142 × AU/mL), and the results of the present study have been expressed in BAU/mL. The cut-off of 7.1 BAU/mL was used to define seropositivity.
5
Serological and Molecular Screening for HBV and HCV
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The serum samples were screened for HBV and HCV serological markers using the Architect i1000SR immunoassay analyzer (Abbott, Abbott Park, IL, USA), following the instructions of the manufacturer. An ARCHITECT HBsAg Qualitative II Reagent kit, ARCHITECT Anti-HBs reagent kit, ARCHITECT Anti-HBc II Reagent kit and ARCHITECT Anti-HCV Reagent kit were used to perform these assays. All these analytical kits for the serological markers were procured from Abbott (Abbott Park, IL, USA). A reading equal to or greater than 1.00 was considered reactive, whereas less than 1.00 was considered non-reactive.
For screening with a nucleic acid amplification test (NAT), HBV and HCV nucleic acid quantification and detection were carried out for all plasma samples using CobasTaqScreenMPX Test, version 2.0 (Roche molecular diagnostics, Basel, Switzerland). The MPX assay was run on a Cobas s 201 system (Roche molecular diagnostics, Basel, Switzerland), as per the manufacturer’s instructions.
For screening with a nucleic acid amplification test (NAT), HBV and HCV nucleic acid quantification and detection were carried out for all plasma samples using CobasTaqScreenMPX Test, version 2.0 (Roche molecular diagnostics, Basel, Switzerland). The MPX assay was run on a Cobas s 201 system (Roche molecular diagnostics, Basel, Switzerland), as per the manufacturer’s instructions.
6
Tacrolimus Variability in Lung Transplant
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The pertinent parameters were collected through a retrospective chart review of all patients, including demographic data (age, sex, height, weight, comorbidities), laboratory data (alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, serum creatinine, urea, fasting blood glucose, hemoglobin, hematocrit), transplant-related data (indication for transplant, transplant type, use of basiliximab and use of extracorporeal membrane oxygenation (ECMO)), TAC C 0 , Forced expiratory volume in 1 second (FEV1), presence of donor-specific antibodies (DSA) and mortality were obtained. The TAC C 0 values were determined using micro-particle enzyme immunoassay (ARCHITECT i1000SR immunoassay analyzer, Abbott U.S.). All TAC C 0 data within the 0-3 months, 3-12 months, and 12-24 months were included. The average TAC C 0 were calculated for each patient within each respective epoch. TAC IPV was calculated as the coefficient of variation (CV) using the formula TAC IPV = (standard deviation/mean) × 100%, as previously reported. 19 A minimum of three measurements per each post-transplant epoch were required to calculate IPV. Respiratory function test was performed at four weeks post-transplantation and then every 3 months thereafter, or based on physicians' discretion.
7
Kisspeptin-10 and Vitamin D Quantification
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Serum concentrations of kisspeptin-10 were assessed by an immunoenzymatic method with the commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions (Human Kisspeptin-10 Elisa Kit, standard curve range: 0.05 ng/mL-10 ng/mL, sensitivity: 0.02 ng/mL, intra-assay CV: 8%; interassay CV: 10%; catalog no. E3919Hu, Bioassay Technology Laboratory, Shanghai, China). The absorbance of the samples was measured by a microtiter plate reader at 450 nm (ELx800TM, Bio-Tech Instruments, USA), and the levels of kisspeptin-10 were expressed in units of ng/mL. Serum levels of vitamin D were assessed by chemiluminescence immunoassay (CLIA) method with the commercially available Architect 25-OH vitamin D kit and measured by Abbott Architect i1000SR immunoassay analyzer. The levels of vitamin D were also expressed in units of ng/mL.
8
Serum Biomarker Measurement Protocol
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Serum concentrations of CA19-9 were measured by use of a CA19-9 RIA kit (Centocor), which has a recommended upper reference limit of 37 U/mL. CEA concentrations were measured by chemiluminescent microparticle immunoassay with an Architect i1000SR Immunoassay Analyzer (Abbott Laboratories). The cutoff concentrations for CA19-9 and CEA were determined based on known values of 37 U/mL and 5 U/mL, respectively.
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