The mouse dorsal skin sections were stained with the toluidine blue O purchased from Macklin (Shanghai, China). Sections were first washed with PBS twice for 5 min and dried, before incubation in 0.1% toluidine blue staining solution in 1% NaCl solution, pH 2.3, for 5–30 min. Toluidine blue-stained sections were washed with DDI water, twice for 5 min. Differentiation of nuclei and particles were followed by 0.5% glacial acetic acid. Rapid dehydration was subjected with 95% absolute ethanol, followed by dibutyl phthalate in xylene reagent (Sigma-Aldrich) for microscopic evaluation.
Toluidine blue o
Manufactured by Maclin Biochemical Technology
Sourced in China
Toluidine blue O is a metachromatic dye that can be used in various laboratory applications. It is a water-soluble thiazine dye that exhibits a blue-purple color. The dye is commonly used for staining and visualization purposes in microscopy, histology, and cytology.
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2 protocols using toluidine blue o
1
Toluidine Blue Staining of Mouse Skin
2
Moso Bamboo PF Resin Characterization
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Four-year-old Moso bamboo (Phyllostachys pubescens Mazel) was obtained from Huzhou, Zhejiang Province, China. PF resin of 47.5 wt%, the viscosity of 40 cps, Mw of 919, and pH of 10.04 was supplied by the Beijing Dynea Chemical Industry Co., Ltd., Beijing, China. Safranine T (Macklin) and toluidine blue O (Macklin) were used as received.
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