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Hiseq 2000 v3 kits

Manufactured by Illumina
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The HiSeq 2000 v3 kits are a set of consumables designed for use with the HiSeq 2000 sequencing system. The kits include reagents, flow cells, and other components necessary to perform high-throughput DNA sequencing using the Illumina HiSeq 2000 platform.

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Lab products found in correlation

Truseq rna sample preparation kit v2, by Illumina (2 mentions) Trueseq stranded mrna library preparation kit, by Illumina (2 mentions)

Spelling variants of this product

HiSeq 2000 (10114 citations) HiSeq 2000 v3 (8 citations)

2 protocols using hiseq 2000 v3 kits

1

Illumina RNA-Seq Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
mRNA in total RNA was isolated and converted into non-stranded or stranded libraries. Non-stranded libraries were produced manually using reagents provided in the Illumina TruSeq RNA Sample Preparation Kit v2 in accordance with manufacturer’s recommendations. The protocol was modified to produce size-selected libraries by modifying the fragmentation conditions and using a Caliper LabChip XT instrument. Stranded libraries were prepared using a NeoPrep Library Prep System and the reagents provided in the Illumina TrueSeq Stranded mRNA Library Preparation kit. The stranded library prep workflow is similar to the non-stranded workflow, except that it involves additional ribosomal reduction chemistry to maximise the percentage of uniquely mapped reads. Following purification, the RNA was fragmented and synthesised into cDNA using a reverse transcriptase process. The products were then enriched with PCR (maximum 10 cycles) to create the final cDNA library. Enriched libraries were subjected to 75 base paired-end sequencing using Illumina HiSeq 2000 v3 kits following manufacturer’s instructions.
Kilpinen H., Goncalves A., Leha A., Afzal V., Alasoo K., Ashford S., Bala S., Bensaddek D., Casale F.P., Culley O.J., Danecek P., Faulconbridge A., Harrison P.W., Kathuria A., McCarthy D., McCarthy S.A., Meleckyte R., Memari Y., Moens N., Soares F., Mann A., Streeter I., Agu C.A., Alderton A., Nelson R., Harper S., Patel M., White A., Patel S.R., Clarke L., Halai R., Kirton C.M., Kolb-Kokocinski A., Beales P., Birney E., Danovi D., Lamond A.I., Ouwehand W.H., Vallier L., Watt F.M., Durbin R., Stegle O, & Gaffney D.J. (2017). Common genetic variation drives molecular heterogeneity in human iPSCs. Nature, 546(7658), 370-375.
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2

Illumina RNA-Seq Library Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
mRNA in total RNA was isolated and converted into non-stranded or stranded libraries. Non-stranded libraries were produced manually using reagents provided in the Illumina TruSeq RNA Sample Preparation Kit v2 in accordance with manufacturer’s recommendations. The protocol was modified to produce size-selected libraries by modifying the fragmentation conditions and using a Caliper LabChip XT instrument. Stranded libraries were prepared using a NeoPrep Library Prep System and the reagents provided in the Illumina TrueSeq Stranded mRNA Library Preparation kit. The stranded library prep workflow is similar to the non-stranded workflow, except that it involves additional ribosomal reduction chemistry to maximise the percentage of uniquely mapped reads. Following purification, the RNA was fragmented and synthesised into cDNA using a reverse transcriptase process. The products were then enriched with PCR (maximum 10 cycles) to create the final cDNA library. Enriched libraries were subjected to 75 base paired-end sequencing using Illumina HiSeq 2000 v3 kits following manufacturer’s instructions.
Kilpinen H., Goncalves A., Leha A., Afzal V., Alasoo K., Ashford S., Bala S., Bensaddek D., Casale F.P., Culley O.J., Danecek P., Faulconbridge A., Harrison P.W., Kathuria A., McCarthy D., McCarthy S.A., Meleckyte R., Memari Y., Moens N., Soares F., Mann A., Streeter I., Agu C.A., Alderton A., Nelson R., Harper S., Patel M., White A., Patel S.R., Clarke L., Halai R., Kirton C.M., Kolb-Kokocinski A., Beales P., Birney E., Danovi D., Lamond A.I., Ouwehand W.H., Vallier L., Watt F.M., Durbin R., Stegle O, & Gaffney D.J. (2017). Common genetic variation drives molecular heterogeneity in human iPSCs. Nature, 546(7658), 370-375.
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