Cs5522a

Manufactured by Pentax

The CS5522A is a Pentax laboratory equipment product. It is a precision instrument designed for scientific and technical applications. The core function of the CS5522A is to provide accurate and reliable measurements, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Eswab, by Copan (2 mentions) Nutriselect plus, by Merck Group (1 mentions) Dey engley broth, by Merck Group (1 mentions)

3 protocols using cs5522a

Sampling Methods for Medical Device Decontamination

Cited 1 time

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The sampling method was based on the FDA/CDC/ASM
11
and Dutch guidelines
12
and described in our previous study
10 (link)
. Samples of the distal tip and working/suction channel were acquired immediately after decontamination and after overnight storage in the PlasmaBAG. Two samples were collected at each sampling moment: 1) a swab sample (eSwab, COPAN, Brescia, Italy) from the distal tip; and 2) a flush-brush-flush (FBF) sample from the suction and working channel. The FBF sample consisted of two flushes with 20 mL of sterile water and a brush of the channels (CS5522A, Pentax Medical, Dodewaard, The Netherlands). A neutralizing solution (Dey-Engley broth, NutriSelect Plus, Merck KGaA, Darmstadt, Germany) was added in a 1:1 ratio to both samples. The swab solution was poured onto a tryptic soy agar plate and the FBF sample was filtered and placed on an R2A agar plate. After 3 days of incubation at 35 °C, colony-forming units (CFUs) were counted per sample with a maximum of 100 CFUs/sample.

Kwakman J.A., Vos M.C, & Bruno M.J. (2023). Investigation of the efficacy of an innovative endoscope drying and storage method in a simulated ERCP setting. Endoscopy International Open, 11(4), E419-E425.

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Microbiological Sampling of Endoscopes

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Directly after HLD and again after overnight storage, two samples were taken for culture. A sample of the distal tip was collected using a flocked eSwab with accompanying Amies fluid (eSwab; Copan, Brescia, Italy)
7
. A flush-brush-flush sample of the suction/working channel was collected using two flushes of 20 mL sterile water and a disposable brush (CS5522A; Pentax). Then 40 mL of neutralizer (Dey-Engley broth; Merck KGaA, Darmstadt, Germany) was added to the channel sample
8
and 1 mL to the eSwab sample. After it had been vortexed, the 2-mL eSwab medium was poured onto tryptic soy agar plates. The flush samples were filtered through a 0.45-µm filter, which was placed on R2A agar. Samples were incubated at 35 °C and examined for growth after 72 hours.
Culture results were presented in CFUs per microorganism per sample site, with a maximum of 100 CFUs per sample. To differentiate between growth of Pa-type 1 and Pa-type 2 in phase 2, matrix-assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) combined with cluster analysis was used (
Appendix 1 s, see online-only Supplementary material).

Kwakman J.A., Bexkens M.L., Bruno M.J, & Vos M.C. (2022). Persistent contamination of a duodenoscope working channel in a non-clinical simulated ERCP setting. Endoscopy, 54(11), 1085-1090.

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Duodenoscope Culture Sampling Protocol

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The duodenoscope culture sets consisted of five sample sites. First, the distal tip of the duodenoscope was swabbed using a Copan Liquid Amies Elution Swab (eSwab; Copan). Then 20 mL of sterile saline (0.9%) was flushed through each of the suction channel, biopsy channel, and air water channel and collected separately in sterile containers. Subsequently, a single-use endoscope cleaning brush (Pentax CS5522A) was pulled through the suction and biopsy channels. The distal tip of the brush was cut using disinfected pliers and placed in an eSwab container.
Starting in April 2021, sterile water was used as the flushing fluid instead of saline. Routine surveillance cultures were taken approximately monthly. Data on the exact timing of sampling and errors in the sampling process were not available.

van der Ploeg K., Haanappel C.P., Voor In 't Holt A.F., de Groot W., Bulkmans A.J.C., Erler N.S., Mason-Slingerland B.C.G.C., Vos M.C., Bruno M.J, & Severin J.A. (2024). Effect of a novel endoscope cleaning brush on duodenoscope contamination. Endoscopy, 56(3).

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