SMARTpool small interfering RNA (siRNA) reagents, including control (D-001810–10) and PP2A-C (L-003598–01) were purchased from Dharmacon (GE; Lafayette, CO). The SET short hairpin RNA (shSET) were obtained from National RNAI Core Facility Platform (Taiwan). In brief, cells were first transfected with siRNA (final concentration, 100 nM) using the Dharma-FECT4 transfection reagent (Dharmacon) according to the manufacturer's instructions for 48 hours. The efficacy of genetic knockdown was confirmed by western blot.
Control d 001810 10
Manufactured by Horizon Discovery
Sourced in United States
Control (D-001810–10) is a lab equipment product provided by Horizon Discovery. It serves as a standard reference material for comparison and validation purposes in various experimental contexts. The core function of this product is to establish a baseline or control condition against which experimental results can be measured and evaluated.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using control d 001810 10
1
Silencing PP2A-C and SET in Cells
2
Targeted knockdown and apoptosis analysis
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SMARTpool small interfering RNA (siRNA) reagents, including control (D-001810-10) and PP2A-C (L-003598-01) were purchased from Dharmacon (Chicago, IL, USA) and the experiments were performed as previously described.22 (link) In brief, cells were first transfected with siRNA (final concentration, 100 nM) in six-well plates using the Dharma-FECT4 transfection reagent (Dharmacon) according to the manufacturer's instructions for 48 h. Afterward, the medium was replaced and the cells were treated with TD52 (2 μM for 24 h) and then harvested for western blot analysis and apoptosis analysis by flow cytometry.
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