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Pdcd1 is a protein that plays a key role in the regulation of the immune system. It functions as an immune checkpoint regulator, modulating the activity of T cells. Pdcd1 is involved in the maintenance of immune system homeostasis and the prevention of autoimmune diseases.

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Lab products found in correlation

High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (4 mentions) Quantstudio 7 flex system, by Thermo Fisher Scientific (2 mentions) Ctla 4, by Thermo Fisher Scientific (2 mentions) Rneasy micro kit, by Qiagen (2 mentions)

3 protocols using pdcd1

1

Simultaneous RNA and Protein Detection

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The PrimeFlow RNA Assay is an in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using fluorochrome-conjugated antibodies. Genes related to T cell persistence (telomerase Tert, IL-2), memory (KLF2, TCF1, Lef1), homing (CCR7, ITAG5, ITAG3) and activation/exhaustion (PDCD1) were included following instructions from the manufacturer (Thermo Fisher Scientific). The gene/protein expression of stimulated cells that were double positive for surface EGFR and intracellular IFNγ by flow cytometry (10,000 cells were acquired) were analyzed.
Wang X., Urak R., Walter M., Guan M., Han T., Vyas V., Chien S.H., Gittins B., Clark M.C., Mokhtari S., Cardoso A., Diamond D.J., Zaia J., Forman S.J, & Nakamura R. (2022). Large-scale manufacturing and characterization of CMV-CD19CAR T cells. Journal for Immunotherapy of Cancer, 10(1), e003461.
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2

Gene Expression Analysis by qRT-PCR

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RNA was isolated using the RNeasy Micro Kit (Qiagen 74004) following the manufacturer’s instructions. RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific 4368813) according to manufacturer’s instructions. Real-time PCR was performed on the QuantStudio 7 Flex System (Applied Biosystems) using the following probes: Actb (Mm 00697939), Pdcd1 (Mm 00435532), Ctla4 (Mm 00486849), Icos (Mm 00497600) and Fasn (Mm01253304) (all from Thermo Fisher Scientific). The primers for SYBR Green master mix were Hmgcr (forward: 5’-AGCTTGCCCGAATTGTATGTG-3’, reverse: 5’-TCTGTTGTGAACCATGTGACTTC-3’), Scap (forward: 5’- TCCAAACACCGGACCGC-3’, reverse: 5’-AGGGTCATCCTCGGCTAAGT
−3’), and Actb (forward: 5’-GACAGGATGCAGAAGGAGATTACTG-3’, reverse: 5’-GCTGATCCACATCTGCTGGAA-3’).
Lim S.A., Wei J., Nguyen T.L., Shi H., Su W., Palacios G., Dhungana Y., Chapman N.M., Long L., Saravia J., Vogel P, & Chi H. (2021). Lipid signalling enforces Treg cell functional specialization in tumours. Nature, 591(7849), 306-311.
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3

Gene Expression Analysis by qRT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated using the RNeasy Micro Kit (Qiagen 74004) following the manufacturer’s instructions. RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific 4368813) according to manufacturer’s instructions. Real-time PCR was performed on the QuantStudio 7 Flex System (Applied Biosystems) using the following probes: Actb (Mm 00697939), Pdcd1 (Mm 00435532), Ctla4 (Mm 00486849), Icos (Mm 00497600) and Fasn (Mm01253304) (all from Thermo Fisher Scientific). The primers for SYBR Green master mix were Hmgcr (forward: 5’-AGCTTGCCCGAATTGTATGTG-3’, reverse: 5’-TCTGTTGTGAACCATGTGACTTC-3’), Scap (forward: 5’- TCCAAACACCGGACCGC-3’, reverse: 5’-AGGGTCATCCTCGGCTAAGT
−3’), and Actb (forward: 5’-GACAGGATGCAGAAGGAGATTACTG-3’, reverse: 5’-GCTGATCCACATCTGCTGGAA-3’).
Lim S.A., Wei J., Nguyen T.L., Shi H., Su W., Palacios G., Dhungana Y., Chapman N.M., Long L., Saravia J., Vogel P, & Chi H. (2021). Lipid signalling enforces Treg cell functional specialization in tumours. Nature, 591(7849), 306-311.
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