Cells were lysed by Laemmli Sample Buffer. Lysates were boiled at 100°C for 5–10 min and separated in 4–12% SDS-PAGE gels (NuPAGE Invitrogen). After transferring to nitrocellulose membranes (Amersham Protan), blots were blocked and incubated with the indicated primary antibodies. Antibody bindings were detected by incubating with IRDye®-Conjugated secondary antibody (LI-COR). The primary antibodies used were anti-TRF1 (1:1000; BED5, Bio-Rad), anti-TERT (1:1000; ab320320, abcam), anti-NANOG (1:1000; Cell Signaling, 4903), anti-OCT4 (1:1000; Cell Signaling, 2750), anti-SOX2 (1:1000; Cell Signaling, 3579), and anti-β-Action (1:500; Santa Cruz 47,778).
Anti β action
Manufactured by Santa Cruz Biotechnology
Anti-β-Action is a laboratory reagent used to detect and quantify the presence of the beta-actin protein in cellular samples. It functions as a specific antibody that binds to the beta-actin protein, enabling its identification and measurement.
Automatically generated - may contain errors
2 protocols using anti β action
1
Protein Expression Analysis in Stem Cells
2
Protein Expression Analysis in Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed by Laemmli Sample Buffer. Lysates were boiled at 100°C for 5–10 min and separated in 4–12% SDS-PAGE gels (NuPAGE Invitrogen). After transferring to nitrocellulose membranes (Amersham Protan), blots were blocked and incubated with the indicated primary antibodies. Antibody bindings were detected by incubating with IRDye®-Conjugated secondary antibody (LI-COR). The primary antibodies used were anti-TRF1 (1:1000; BED5, Bio-Rad), anti-TERT (1:1000; ab320320, abcam), anti-NANOG (1:1000; Cell Signaling, 4903), anti-OCT4 (1:1000; Cell Signaling, 2750), anti-SOX2 (1:1000; Cell Signaling, 3579), and anti-β-Action (1:500; Santa Cruz 47,778).
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