Thawed PBMCs from patients and controls were washed with PBS 1× and blocked with FcR Blocking Reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) for 10 min at room temperature. Cells were then incubated for at least 30 min at 4 °C, protected from light, with the antibodies described below. For T blasts, the following antibodies were used: anti-TCRαβ-BV421 (Biolegend, San Diego, CA, USA, #306722), anti-CD4-FITC (Biolegend, #344604), anti-CD8-BV510 (Sony Biotechnology, San Jose, CA, USA #2323660), anti-CD95-BV711 (Biolegend, #305644), anti-HLA-DR-PC7 (Biolegend, #307616), anti-CD45RA-APC (Biolegend, #304112). For B-LBL: anti-CD19-FITC (BD, #555412), anti-CD95-BV711 (Biolegend, #305644), anti-HLA-DR-PC7 (Biolegend, #307616). Surface staining was detected by LSRFortessaTM X-20 SORP Cell Analyzer (BD) and analyzed with FlowJo (BD; v10.7).
Consonni F., Moreno S., Vinuales Colell B., Stolzenberg M.C., Fernandes A., Parisot M., Masson C., Neveux N., Rosain J., Bamberger S., Vigue M.G., Malphettes M., Quartier P., Picard C., Rieux-Laucat F, & Magerus A. (2024). Study of the potential role of CASPASE-10 mutations in the development of autoimmune lymphoproliferative syndrome. Cell Death & Disease, 15(5), 315.
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