Pd l1 antibody

Manufactured by R&D Systems

Sourced in United States

The PD-L1 antibody is a laboratory reagent used in research applications. It binds to the PD-L1 protein, which is a key regulator of the immune system. The antibody can be used in various experimental techniques, such as flow cytometry, immunohistochemistry, and western blotting, to study the expression and function of PD-L1 in different biological systems.

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Lab products found in correlation

Ihc kit, by Santa Cruz Biotechnology (2 mentions) Hematoxylin, by Vector Laboratories (1 mentions) Deadend fluorometric tunel assay kit, by Promega (1 mentions) Igepal co 520, by Merck Group (1 mentions) Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (1 mentions) Cyclohexane, by Merck Group (1 mentions) 1 2 dioleoyl 3 trimethylammonium propane chloride salt dotap, by Avanti Polar Lipids (1 mentions) Ly294002 hydrochloride, by Merck Group (1 mentions) β galactosidase, by Meridian Bioscience (1 mentions) Stattic, by Merck Group (1 mentions)

4 protocols using pd l1 antibody

Immunohistochemical Analysis of PD-L1

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Tissues obtained were fixed in 10% (v/v) formaldehyde in PBS, embedded in paraffin, and cut into 5-μm sections. Tumor tissue sections were deparaffinized in xylene solution, rehydrated, and immunostained with the IHC kit (Santa Cruz, sc2018). PD-L1 antibody was obtained from R&D (MAB1086). After staining, tissues were counterstained by Hematoxylin (Vector Laboratories, H3401). The average numbers of positively stained cells were obtained from careful counting of randomly chosen 3 different fields.

Shen M.J., Xu L.J., Yang L., Tsai Y., Keng P.C., Chen Y., Lee S.O, & Chen Y. (2017). Radiation alters PD-L1/NKG2D ligand levels in lung cancer cells and leads to immune escape from NK cell cytotoxicity via IL-6-MEK/Erk signaling pathway. Oncotarget, 8(46), 80506-80520.

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Synthesis and Characterization of Lipid-Based Nanoparticles

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GMP disodium salt was synthesized by HDH Pharm Inc (Morrisville, NC). 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP), 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA), 1,2-distearoryl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol-2000) ammonium salt (DSPE-PEG₂₀₀₀) and 25-[N-[(7-nitro-2–1,3-benzoxadiazol-4-yl)methyl]amino]-27-norcholesterol (25-NBD cholesterol) were purchased from Avanti Polar Lipids (Alabaster, AL). Cholesterol, cyclohexane and Igepal CO-520 were from Sigma-Aldrich (St. Louis, MO). DeadEnd Fluorometric TUNEL assay kit was obtained from Promega Corporation (Madison, WI). PD-L1 antibody was purchased from R&D System. Alexa Fluor 647 Phalloidin was obtained from Thermo Fisher Scientific. Other chemicals, unless otherwise specified, were from Sigma-Aldrich (St. Louis, MO). Fluorescent antibodies against mouse CD4 (clone GK1.5), NK1.1 (clone PK136), IFN-γ (clone XMG1.2), TNF-α (clone MP6-XT22), CD16/32 (clone 93), CD25 (clone PC61.5), CD11c (clone N418) and Foxp3 were from eBioscience. Fluorescent antibodies against mouse CD8a (clone 53–6.7), CD11b (clone M1/70), Ly6C (clone HK1.4), Ly6G (clone 1A8), F4/80 (clone BM8), CD80 (clone 16–10A1), CD206 (clone C068C2) were from Biolegend (San Diego, CA).

Zhang Y., Bush X., Yan B, & Chen J.A. (2018). Gemcitabine nanoparticles promote antitumor immunity against melanoma. Biomaterials, 189, 48-59.

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Recombinant HCV Protein and Immune Modulators

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Recombinant HCVc (aa 2–192 of the HCV polyprotein) and β-galactosidase were purchased from Meridian Life Science, Inc. (Saco, ME, USA) and the purifications were greater than 95%; Human TLR2 antibody, PD-L1 antibody and 1-methyl-DL-tryptophan (1-MT) were purchased from R&D; Human IL-10 antibody was purchased from eBioscience; LY-294002 hydrochloride and Stattic were obtained from Sigma-Aldrich.

Zhai N., Li H., Song H., Yang Y., Cui A., Li T., Niu J., Crispe I.N., Su L, & Tu Z. (2017). Hepatitis C Virus Induces MDSCs-Like Monocytes through TLR2/PI3K/AKT/STAT3 Signaling. PLoS ONE, 12(1), e0170516.

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Immunohistochemical Analysis of PD-L1 in Tumor Xenografts

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Tumor tissues obtained from xenografts were fixed in 10% (v/v) formaldehyde in PBS, embedded in paraffin, and cut into 5-µm sections. Tumor tissue sections were deparaffinized in xylene solution, rehydrated, and immunostained with the IHC kit (Santa Cruz, SC2018) and stained for PD-L1 using PD-L1 antibody (R&D, MAB1086). After staining, tissues were counterstained by Hematoxylin. After staining, three areas were randomly selected from slides of three different stains by an investigator not involved in this study, and positive stained cell numbers were obtained.

Yang L., Shen M., Xu L.J., Yang X., Tsai Y., Keng P.C., Chen Y, & Lee S.O. (2017). Enhancing NK cell-mediated cytotoxicity to cisplatin-resistant lung cancer cells via MEK/Erk signaling inhibition. Scientific Reports, 7, 7958.

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