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Fcs express 5 image cytometry

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FCS Express 5 Image Cytometry is a software application designed for the analysis and visualization of flow cytometry and microscopy data. It provides tools for data management, gating, and statistical analysis.

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Lab products found in correlation

Aperio imagescope at, by Leica (1 mentions)

Close spelling variants of this product

FCS Express (241 citations) FCS Express 5 (94 citations)

2 protocols using fcs express 5 image cytometry

1

Multiplex IHC imaging and analysis pipeline

Cited 1 time
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Chromogenic sequential multiplex IHC and digital image acquisition were conducted as described before (8 (link)). Briefly, 5 μm of formalin fixed paraffin-embedded tissue sections were stained by hematoxylin, followed by whole-tissue scanning using Aperio ImageScope AT (Leica Biosystems). Following endogenous peroxidase blocking and heat-mediated antigen retrieval, sequential IHC consisting of iterative cycles of staining, scanning, and antibody and chromogen stripping was performed. Acquired digital images were co-registered so that cell features overlap down to a single-pixel level, using CellProfiler pipeline (8 (link), 19 ). Then, pixel intensity and shape-size measurements were saved to a file format compatible with image cytometry data analysis software, FCS Express 5 Image Cytometry v.5.01.0029 (De Novo Software) (8 (link)).
Tsujikawa T., Thibault G., Azimi V., Sivagnanam S., Banik G., Means C., Kawashima R., Clayburgh D.R., Gray J.W., Coussens L.M, & Chang Y.H. (2019). Robust cell detection and segmentation for image cytometry reveal Th17 cell heterogeneity#. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(4), 389-398.
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2

Single-Cell Image Cytometry Analysis

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Single-cell segmentation and quantification of staining intensity was performed using a CellProfiler v.2.1.1 pipeline. Pixel intensity and shape-size measurements were compatibility with image cytometry data analysis software, FCS Express 5 Image Cytometry v.5.01.0029 (De Novo Software) used for setting gate thresholds for subsequent analysis.
Rambow F., Rogiers A., Marin-Bejar O., Aibar S., Femel J., Dewaele M., Karras P., Brown D., Chang Y.H., Debiec-Rychter M., Adriaens C., Radaelli E., Wolter P., Bechter O., Dummer R., Levesque M., Piris A., Frederick D.T., Boland G., Flaherty K.T., van den Oord J., Voet T., Aerts S., Lund A.W, & Marine J.C. (2018). Toward Minimal Residual Disease-Directed Therapy in Melanoma. Cell, 174(4).
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