The DPPH scavenging capacity was measured as previously described [28 (link)] with slight modifications. In brief, the sample (100 μl) was added into 100 μl of DPPH solution (0.5 mM DDPH solution diluted in 95% ethanol) and incubated in a 96-well plate at room temperature for 30 min. The sample absorbance (A), ethanol absorbance (B) or control absorbance (C) were measured at 517 nm. The DPPH scavenging capacity was calculated using the following formula:
The ABTS kit instructions were followed for the ABTS assay (Beyotime, China) [29 ]. Briefly, ABTS solution (200 μl) and sample (10 μl) were added to each well of a 96-well plate. The plate was gently mixed and incubated at room temperature for 5 min. The sample absorbance (A), ethanol absorbance (B) and control absorbance (C) were measured at 734 nm. The ABTS scavenging capacity was calculated using the above formula. All experiments were performed in at least four parallels and repeated three times.
The ABTS kit instructions were followed for the ABTS assay (Beyotime, China) [29 ]. Briefly, ABTS solution (200 μl) and sample (10 μl) were added to each well of a 96-well plate. The plate was gently mixed and incubated at room temperature for 5 min. The sample absorbance (A), ethanol absorbance (B) and control absorbance (C) were measured at 734 nm. The ABTS scavenging capacity was calculated using the above formula. All experiments were performed in at least four parallels and repeated three times.