Fetal bovine serum, penicilin-streptomycin, Lipofectamine 2000, goat anti-mouse antibody coupled to Alexa 594 and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Invitrogen (Carlsbad, CA, USA). Bovine seroalbumin, paraformaldehyde, phosphate buffered saline (PBS) and the protease inhibitors cocktail were from Sigma Chemical Co. (St. Louis, MO, USA). Mouse anti Rab4, Rab11 or anti-CAV-1 antibodies were purchased from BD Biosciences. LysoTracker Red was from Molecular Probes. Ang-(1-7) was from Bachem. The plasmid containing MAS1R-YFP cDNA was obtained as previously described.17 (link) shRNA ß-arrestin2 was from SantaCruz. All other chemicals were analytical grade reagents of the highest purity available.
Goat anti mouse antibody coupled to alexa 594
Manufactured by Thermo Fisher Scientific
Sourced in United States
Goat anti-mouse antibody coupled to Alexa 594 is a fluorescent-labeled secondary antibody used for the detection and visualization of mouse primary antibodies in various immunological and cell biology applications. It consists of a goat-derived antibody that specifically binds to mouse immunoglobulins, conjugated with the Alexa Fluor 594 fluorescent dye.
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Lab products found in correlation
Protease inhibitors cocktail, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicilin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Bovine seroalbumin, by Merck Group (1 mentions)
Lysotracker red, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Ang 1 7, by Bachem (1 mentions)
Goat anti rabbit antibody coupled to alexa488, by Thermo Fisher Scientific (1 mentions)
2 protocols using goat anti mouse antibody coupled to alexa 594
1
Analyzing Angiotensin Receptor Trafficking
2
Proteasome Activity Assay in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fetal bovine serum, penicilin-streptomycin, goat anti-mouse antibody coupled to Alexa 594, goat anti-rabbit antibody coupled to Alexa 488 and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Invitrogen (Carlsbad, CA, USA). Rabbit anti-Ub antibody was from Santa Cruz Biotechnology Laboratories (Santa Cruz, CA, USA). Bovine seroalbumin (BSA), paraformaldehyde (PFA 4%), phosphate buffered saline (PBS) and the protease inhibitors cocktail were from Sigma Chemical Co. (St. Louis, MO, USA). Polyclonal anti-TH rabbit, monoclonal anti-TH mouse, anti-TH phosphoSer-19 rabbit and anti-TH phosphoSer-40 rabbit antibodies were from Chemicon (San Francisco, CA, USA). Phosphatase inhibitor, ubiquitinated protein enrichment kit, proteasome substrate III Suc-Leu-Leu-Val-Tyr-aminomethyl coumarin (Suc-LLVY-AMC) and monoclonal anti-20S proteasome alfa1,2,3,5,6&7-subunits mouse antibody were from Calbiochem (EMD Chemicals, Gibbstown, USA).
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