Affinity purified f ab 2 fragments of polyclonal goat anti mouse igm anti ig

Affinity-purified F(ab′)₂ fragments of polyclonal goat anti-mouse IgM (anti-Ig), and HRP-conjugated goat anti-rabbit secondary antibodies, were obtained from Jackson ImmunoResearch Laboratories. Anti-phospho-PKCδ (pTyr311), anti-PKCδ, anti-phospho-Lyn (pTyr507), anti-Lyn, anti-phospho-Fyn (pTyr530), anti-Fyn, anti-phospho-Blk (pTyr389), anti-Blk, anti-phospho-Syk (pTyr352), anti-Syk, anti-phospho-Src (pTyr416), anti-Src anti-JAK1, anti-JAK3, anti-GAPDH, and anti-actin Abs for immunoblotting were obtained from Cell Signaling Technology. Anti-phospho-Lyn (pTyr396) was obtained from Abcam. Protein A/G ultralink resin was obtained from Thermo Scientific. LY294002, was obtained from Calbiochem. PP2 and PP3 were obtained from abcam. SU6656 was obtained from Santa Cruz Biotechnology. Recombinant murine IL-4 and goat anti-IL-4Rα antibody was obtained from R&D Biosystems. HRP-conjugated rabbit anti-goat secondary antibody, was obtained from southern biotech.

Affinity-purified F(ab′)2 fragments of polyclonal goat anti-mouse IgM (anti-Ig) and polyclonal goat anti-rabbit Ab-APC were obtained from Jackson ImmunoResearch Laboratories. Anti-mouse mAbs against CD19-APC, B220-PE, B220-FITC, CD5-PE-Cy7, CD43-PE, PD-L2-PE, and PD-L2-APC, CD86-PE, CD44-PE, CD9-PE, CD80-PE, Thy-1-PE, CD45.1-FITC, phospho-p38, and phospho-ERK-APC were obtained from BD pharmingen. Polyclonal anti-pERK, anti-NFATc1, anti-RasGRP3, and anti-phospho-JNK antibodies were obtained from Cell Signaling Technology. Anti-RasGRP1 antibody was obtained from Santa Cruz Biotechnology. Phorbol ester myristate (PMA), RIPA buffer, phenylmethylsulfonyl fluoride (PMSF), and protein inhibitor cocktails were obtained from Sigma Aldrich.