Three μm thick paraffin sections were stained for the published HPC markers: panCK, A6 kindly provided by Dr V. Factor (National Cancer Institute, Bethesda, MD), Sox9, CD24, Dlk1, EpCAM, and Fn14 or for the alternate antigens: BrdU, (AB6326 Abcam, UK), p53 (MS 104-P1, LabVision), Ki67, and CYP2D6 (Generous gift from Prof R Wolfe, University of Dundee); see Supplementary Table 2 for additional details. Cytospins and cells were fixed in cold methanol at 4°C for 5 minutes prior to staining Species isotype (Santa Cruz) staining controls were routinely performed. Detection was performed with DAB (DAKO) followed by counterstaining with Haematoxylin or alternately with Alexa 488, 555 or 650 (A21206, A21434A21436/S32355, and A21448 respectively; Invitrogen, UK) with a DAPI containing Vectasheld mounting media (Vector, UK). Apoptosis was assessed using the DeadEnd™ Colorimetric or Fluorometric (Alexa 488) TUNEL System (Promega #TB199) according to manufacturer's instructions.
Species isotype
Manufactured by Santa Cruz Biotechnology
Species isotype is a type of laboratory equipment used for the identification and characterization of antibodies. It functions to determine the class or subclass of an antibody based on its heavy chain constant region. This information is crucial for various immunological applications, such as immunoassays and antibody purification.
Automatically generated - may contain errors
Lab products found in correlation
3 3 diaminobenzidine (dab), by Agilent Technologies (2 mentions)
A21206, by Thermo Fisher Scientific (2 mentions)
Haematoxylin, by Thermo Fisher Scientific (2 mentions)
A21448, by Thermo Fisher Scientific (2 mentions)
A21434a21436 s32355, by Thermo Fisher Scientific (2 mentions)
Vectasheld mounting media, by Vector Laboratories (2 mentions)
2 protocols using species isotype
1
Immunohistochemical Analysis of Liver Progenitors
2
Immunohistochemical Analysis of Liver Progenitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three μm thick paraffin sections were stained for the published HPC markers: panCK, A6 kindly provided by Dr V. Factor (National Cancer Institute, Bethesda, MD), Sox9, CD24, Dlk1, EpCAM, and Fn14 or for the alternate antigens: BrdU, (AB6326 Abcam, UK), p53 (MS 104-P1, LabVision), Ki67, and CYP2D6 (Generous gift from Prof R Wolfe, University of Dundee); see Supplementary Table 2 for additional details. Cytospins and cells were fixed in cold methanol at 4°C for 5 minutes prior to staining Species isotype (Santa Cruz) staining controls were routinely performed. Detection was performed with DAB (DAKO) followed by counterstaining with Haematoxylin or alternately with Alexa 488, 555 or 650 (A21206, A21434A21436/S32355, and A21448 respectively; Invitrogen, UK) with a DAPI containing Vectasheld mounting media (Vector, UK). Apoptosis was assessed using the DeadEnd™ Colorimetric or Fluorometric (Alexa 488) TUNEL System (Promega #TB199) according to manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!