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Vitek 2 gn id

Manufactured by bioMérieux
Sourced in France

The Vitek 2 GN ID is a microbiological identification system used to identify Gram-negative bacteria. It is a rapid automated system that utilizes standardized identification cards to perform biochemical tests and provide accurate bacterial identification results.

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5 protocols using vitek 2 gn id

1

Screening and Genotyping of ESBL-E

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Screening swabs (soaked with Amies transport medium) were taken from the rectum and cultivated on ChromID ESBL agar (bioMérieux, Nürtingen, Germany) selecting for ESBL-E. Species identification and antibiotic susceptibility testing of bacteria grown on ChromID ESBL agar was performed by Vitek 2 GN ID and AST N223 card (bioMérieux), respectively. Isolates were included in the study, if they tested non-susceptible to cefotaxime, ceftriaxone or ceftazidime using EUCAST breakpoints [24 ].
The combination disc test following EUCAST guidelines using cefotaxime, ceftazidime and cefepime with and without clavulanate (Mast Diagnostica, Reinfeld, Germany) was performed to confirm ESBL production [24 ].
Genotyping of 3GCREB isolates was conducted by repetitive-sequence-based PCR and subsequent microfluidics electrophoresis using the DiversiLab system (bioMérieux).
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2

Antibiotic Resistance in Colombian Isolates

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A total of 60 clinical strains were tested in this study: 30 each for K. pneumoniae and P. aeruginosa. All clinical isolates were isolated from clinical specimens, such as urine, secretions, and blood, recovered from two tertiary care hospitals in Cali, Colombia, between 2017 and 2019. All cultures of clinical isolates were sent to Microbiology Laboratory at Laboratorio de Salud Pública Departamental del Valle del Cauca (LSPD-Valle), where bacterial identity was confirmed, and antibiotic susceptibility characterization was performed. Species identification was performed using the automated VITEK® 2 system, (bioMerieux, 9.02, Marcy l’Etoile, France) with the VITEK® 2 Gram-Negative Identification card (VITEK® 2 GN ID), which is based on established biochemical methods and substrates that evaluate the use of carbon, enzymatic activity, and resistance (Ref. 21341, bioMerieux, Marcy l’Etoile, France). All laboratory strains, including E. coli ATCC® 25922™, K. pneumoniae ATCC® 2146™, and P. aeruginosa ATCC® 27853™, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).
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3

CRAB-cc Isolation and Characterization Protocol

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A. baumannii-calcoaceticus complex isolates were obtained as a part of routine diagnostic procedure and analyzed anonymously. Only one clinical culture with A. baumannii-calcoaceticus complex per patient was included in this study. The strains were isolated and identified from blood, urine, the lower respiratory tract, and wounds based on clinically indicated cultures in each hospital involved in this study.
The identification and antibiotic susceptibility test of CRAB-cc were performed by the VITEK2® system (VITEK® 2 GN ID and VITEK® 2 AST-GN93; bioMérieux, Lyon, France) [19 (link)] and subsequently analyzed according to CLSI 2019 guideline [33 (link)]. The CRAB-cc isolates were stored in trypticase soy broth with 10% glycerin and stored at −80 °C until further characterization.
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4

Comparative Analysis of E. coli Isolates

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The collection of E. coli isolates was performed between 02 October 2016 and 02 June 2017 at King Khaled University Hospital. Throughout this period, 227 wound infections samples were cultivated and identified agreeing with the directions of manufacturer using the full automatic system (Vitek 2 system). The types of wound infection and antibiotic resistance patterns were analyzed and compared with these data that obtained from bacterial isolates obtained from raw milk. The total number of milk samples collected in this study were 240. During the same time, the raw milk samples were collected weekly in a sterilized container at random from one of the companies producing milk in Riyadh, Saudi Arabia, identified here as company R. MacConkey agar (MCA), nutrient agar (NA), blood agar (BA), and violet red bile agar (VRBA) (Oxoid, UK) were used to isolate the bacterial strains (E. coli) based on culture characteristics. The primary identification was carried out using API 20 E (bioMerieux) and the identification was completed using the Vitek ®2 GN ID.
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5

Identification and Antimicrobial Susceptibility of Isolates

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The primary isolation was carried according to the laboratory standard protocol depending on the type and site of the sample on Cysteine lactose electrolyte deficient medium (CLED), blood agar, Mac Conkey agar, and chocolate agar. Clinically significant colonies were identified by using a set of in house biochemical tests as per standard protocols [11] and Vitek 2 GN ID (BioMerieux, France) for isolates from ICU sample with appropriate quality control [12] .
Antimicrobial susceptibility testing was performed using the Clinical and Laboratory Standards Institute (CLSI) disc diffusion method with Mueller-Hinton agar (MHA) (Hi-Media, India). Escherichia coli strain ATCC 25922 were tested as internal control each time performed the susceptibility test [13] .
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