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4 protocols using barium carbonate

1

Synthesis of Perovskite Nanocrystals

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Bulk BP crystal as raw material, which was purchased from Nanjing MKNANO Tech. Co. Ltd (http://www.mukenano.com). PDMS precursor was purchased from Dow Corning. Lead bromide (PbBr2, 99.999%), barium carbonate (Cs2CO3, 99.999%), hexane (anhydrous, 98%), 1-octadecene (ODE, technical grade, 90%), toluene (anhydrous, 98%), oleic acid (OA, technical grade, 90%), oleylamine (OLA, technical grade, 70%), methyl acetate (MeOAc, anhydrous, 99.5%), and N-methyl pyrrolidone (NMP, AR) were purchased from Sigma-Aldrich.
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2

Isotopic Composition Analysis of Barium Compounds

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Table 1 displays the isotopic compositions of 135BaCO3, natBaCO3, and natBa metal. Isotopically enriched 135BaCO3 was obtained from Trace Sciences International. Barium carbonate (99.999% trace metals basis), barium metal (99.99% trace metals basis), American Chemical Society reagent–grade concentrated hydrochloric acid (37%), nitric acid (70%), ammonium hydroxide (28%), and periodic table mix inductively coupled plasma optical emission spectrometry (ICP-OES) elemental standards were obtained from Sigma-Aldrich. Oxalic acid dihydrate (99.5%) was purchased from Fisher Scientific. Aluminum disks were obtained from Michaels, and aluminum foil was purchased from Goodfellow Cambridge. Indium wire was purchased from AIM Specialty Materials. Branched diglycolamide resin was purchased from Eichrom. Eckert and Ziegler Isotopes National Institute of Standards and Technology–traceable γ-ray sources were used for high-purity germanium (HPGe) detector energy and efficiency calibration. Thin-layer chromatography silica gel sheets were purchased from Merck. Water (18 MΩ·cm) was obtained from a MilliporeSigma Direct-Q 3 ultraviolet system. 89Zr was provided by the Washington University Cyclotron Facility. DOTA was purchased from Macrocyclics. Macropa was purchased from MedChemExpress. PSMA-I&T was obtained from ABX Advanced Biochemical Compounds. DCFPyL was synthesized in-house.
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3

Detailed Phytochemical Characterization Protocols

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Malic acid was supplied from Riedel-de Haën (Germany), citric acid anhydrous from J. T. Baker (USA), formic acid from Merck (Sweden), acetic acid from Sigma (USA) and lactic acid was supplied by Acros organics (USA). Galactose and mannose were obtained from Acros organics (USA), glucose from Fisher Scientific (USA), arabinose from Sigma (USA), galacturonic acid and xylose were supplied from Fluka (Slovakia). Sulfuric acid (95-98%) and barium carbonate were purchased from Sigma (USA). Aloin of purity >97% from Aloe barbadensis Miller leaves, 2,2-Diphenyl-1picrylhydrazyl (DPPH), buthylatedhydroxyanisole (BHA), sodium carbonate (Na 2 CO 3 ) and Folin-Ciocalteau (FC) reagent were purchased from Sigma (USA). Ethanol absolute and methanol were obtained from Fisher chemical (UK). Potato dextrose agar (PDA) was purchased from Difco (France) and potato dextrose broth (PDB) from Liofilchem (Italy). All samples, standards and eluents were prepared using demineralized Milli-Q water from Millipore, USA.
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4

Functionalized Lanthanide Nanoparticle Synthesis

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All chemicals were used as received without further purification. Sodium
chloride (NaCl), yttrium nitrate
(Y(NO3)3·6H2O), ytterbium nitrate
(Yb(NO3)3·5H2O), thulium nitrate
(Tm(NO3)3-5H2O), , ammonium fluoride
(NH4F), polyacrylic acid (PAA, MW ~15000), polyacrylic
acid (PAA, MW ~1800), barium carbonate (BaCO3), cupric
chloride (CuCl2), cobalt chloride (CoCl2), zinc sulfate
(ZnSO4), and mercury(II) nitrate monohydrate
(Hg(NO3)2·H2O) were purchased from
Sigma Aldrich (St. Louis, MO). 1-Ethyl-3-[3-dimethylamonopropyl] carbodiimide
hydrochloride (EDC), N-hydroxysuccinimide (NHS) and ethylene
glycol (EG) were purchased from Thermo Scientific (Rockford, IL). SYBR Green I
was from Life Technology (Carlsbad, CA). All DNA strands were from IDT DNA
(Coralville, IA). Nt. Alwl and 10x Cutsmart® buffer were purchased from
New England BioLabs. The melting points of various DNA strands under the
experimental conditions were estimated using the OligoAnalyzer program available
at the vendor’s website (http://www.idtdna.com). The sequences of DNA probes used in this
study are listed in Table 1. The DNA
strands used are DNA_1 (22mer, amine-modified at the 5’-end) and
DNA_mismatch (22mer). The middle segment of DNA_mismatch is complementary to
DNA_1 except at the thymine bases.
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