Qwin 500 image analysis software

Coronal sections from the perfused brain were cut on a microtome (Microm HM 520, Labcon, Germany). The sections were stained with cresyl violet (1%) at 50 °C for 20 min  following dehydration and rehydration through graded series of alcohol following the standard procedure of Gupta et al.⁴⁴ . The sections containing Nissl positive neurons were imaged under light microscopy. The photomicrographs were processed on computerized software that enabled to assess the percent area of a selected field occupied by Nissl stained area. The quantification was performed using the image analysis system (Leica Qwin 500 image analysis software).

Nissl staining was used for histological examination of cell loss. Hippocampal sections from each treatment group were stained with cresyl violet (0.1%), dehydrated through graded series of alcohol and cover slipped with DPX mounting media64 (link). The intensity of Nissl stained positive neurons in dentate gyrus area of hippocampal region was determined using a computerized image analysis system (Leica Qwin 500 image analysis software) as described by Shingo et al.65 (link). The photomicrographs were processed on computerized software that enabled to assess the percent area of a selected field occupied by Nissl stained area.

Immunohistochemical studies were carried out following the method of Ansari et al.53 (link). The intensity of anti-TrkA and anti-p75^NTR in the hippocampal region of the brain was determined using a computerized image analysis system (Leica Qwin 500 image analysis software) as described by Shingo et al.54 (link).