3 mt hcl

Drugs: Methiopropamine hydrochloride (HCl) (mol. wt. 191.7) was purchased from Cayman Chemicals (Middlesex, UK) and (+)methamphetamine HCl (mol. wt. 185.7) was purchased from Chiron AS (Trondheim, Norway). Chemicals: 5-HT HCl, L-NE HCl, 5-HIAA HCl, 3-MT HCl, ephedrine-D₃, sodium fluoride, methanol (LC-MS Chromasolv^®), ammonium formate, benztropine mesylate, reboxetine mesylate hydrate, fluoxetine HCl and adenosine 5′-triphosphate disodium salt hydrate were purchased from Sigma-Aldrich (Oslo, Norway). DA HCl and DOPAC were purchased from Fluka (Buchs, Switzerland). DA-¹³C₆ HCl, 5-HT-D₄ HCl, 5-HIAA-D₅, methamphetamine-¹³C₆ and nor-methiopropamine were purchased from Chiron AS. Di-sodiumtetraborate-10-hydrate and ethyl acetate were purchased from Chemi-Teknik AS (Oslo, Norway). Acetic acid and formic acid were purchased from VWR International AS (Oslo, Norway). Sodium hydroxide and n-heptane were purchased from Merck Millipore (Oslo, Norway). Dihydroxyphenylethylamine 3,4-[ring-2,5,6-³H]-(DA) ([³H]DA), NE HCl DL-[7-³H(N)] ([³H]NE), 5-HT [³H(G)] ([³H]5-HT) and UltimaGold^TM liquid scintillation cocktail were purchased form from PerkinElmer Inc. (Oslo, Norway). HEPES was purchased from Thermo Fischer Scientific (Oslo, Norway) and sodium heparin was purchased from Leo Pharma (Oslo, Norway).

Magnetic nanoparticles (20–30 nm) were purchased from Jinpan biotech (Shanghai, China). HPLC-grade methanol and acetonitrile were purchased from Tedia (Fairfield, OH, USA).
3-methacryloxypropyltrimethoxysilane (MPS), AIBN, MAA and EGDMA were purchased from Aladdin (Shanghai, China).
Creatinine (Cr), dopamine hydrochloride (DA·HCl), DL-E·HCl, DL-NE·HCl, 3-MT·HCl and DL-NMN·HCl were purchased from Sigma-Aldrich (Saint Louis, USA). DL-MN·HCl was purchased from Mucklin (Shanghai, China).
Ethanol, acetic acid, sodium dihydrogen phosphate, disodium hydrogen phosphate, trisodium phosphate and acetic acid were purchased from Chuandong chemical (Chongqing, China), and water was purified by a Milli-Q system from Merck (Darmstadt, Germany). All other chemicals were of analytical grade.