Total protein was extracted from the control and NASH mouse liver with RIPA buffer and PMSF. Protein content was determined using a BCA protein assay reagent. Samples with 30 µg total protein each were separated on 10% SDS PAGE gels and transferred onto polyvinylidene difluoride membranes. The blots were probed with rabbit anti-Cyp1a2 (Proteintech Group, Rosemont, USA), anti-Cyp3a4 (Proteintech Group, Rosemont, USA), anti-Cyp2c19 (Abcam, Cambridge, UK), anti-Cyp2c9 (Bioworld, Bloomington, USA), anti-Cyp2d6 (Proteintech Group, Rosemont, USA), mouse anti-Cyp2e1 (Abcam, Cambridge, UK), and anti-tubulin (Zhengeng, China). Signals were visualized by enhanced chemiluminescence detection.
Anti cyp2d6
Manufactured by Proteintech
Sourced in United States
Anti-Cyp2d6 is a lab equipment product produced by Proteintech. It is an antibody that binds to the Cyp2d6 protein, which is a member of the cytochrome P450 enzyme family. The primary function of Anti-Cyp2d6 is to facilitate the detection and study of the Cyp2d6 protein in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti cyp3a4, by Proteintech (2 mentions)
Mouse anti cyp2e1, by Abcam (1 mentions)
3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Glycogen stain kit, by Nanjing Jiancheng (1 mentions)
Anti hnf4a, by Abcam (1 mentions)
Ta2 fl, by Nikon (1 mentions)
2 protocols using anti cyp2d6
1
Liver Protein Expression Profiling
2
Hepatic Protein Expression Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paraffin-embedded tissue sections (3μm) were deparaffinized and rehydrated in graded alcohol concentrations. After antigen retrieval using sodium citrate buffer, sections were incubated with anti-CYP3A4, anti-CYP2D6, anti-GSTA2 and anti-MRP2 (All from proteintech, USA) and anti-NTCP (Aviva Biosystems, USA) antibodies overnight at 4oC. Stained tissue sections were visualized using HRP Conjugated goat anti-Rabbit IgG (H+L) secondary antibody and 3, 3-diaminobenzidine (DAB) (Both purchased from DAKO, Denmark). For monolayer culture, differentiated and undifferentiated iHepLPCs cells were cultured in 12-well plates and then fixed with 4% Paraformaldehyde. After blocking and permeabilization, cells were incubated with anti-CYP3A4 (proteintech, USA) and anti-HNF4a (Abcam, UK) antibodies overnight at 4oC, and then visualized using Alexa Fluor 488 Conjugated goat anti-Rabbit IgG (H+L) secondary antibody or Alexa Fluor 555 Conjugated Donkey Anti-Mouse IgG(H+L) secondary antibody and 4',6-diamidino-2-phenylindole (DAPI) (Both purchased from Beyotime Biotechnology, China). PAS staining of the monolayer culture was carried using the Glycogen Stain kit (Nanjing Jiancheng Bioengineering Institute), following the instructions provided by the manufacturer. Images were captured with Nikon Ta2-FL.
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