Cells were plated in 96-well plates (in 3 biological replicates for each condition) and infected the next day with virus at the following MOIs: HAV (~5-10 focus forming units/cell), RV-A2 (~1 plaque forming units/cell), EV-71 (~0.2 plaque forming units/cell), EMCV (~0.2 plaque forming units/cell), HCV (~1 focus forming units/cell) and HBV (~1 focus forming units/cell). Cells were harvested, lysates reverse transcribed and quantitative PCR performed on a Bio-Rad CFX96 Touch system using the Power SYBR Cells-to-CT kit (Invitrogen). All viral RNA levels were normalized to 18S levels. Primer sequences can be found in Table S3 .
Cfx96 touch system
Manufactured by Thermo Fisher Scientific
The CFX96 Touch system is a real-time PCR detection system designed for quantitative gene expression analysis. It features a touchscreen interface and a thermal cycler with a 96-well sample block. The system is capable of performing real-time PCR amplification and detection across multiple samples simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cfx96 touch system
1
Quantitative viral RNA analysis
2
Quantitative viral RNA analysis
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