Human embryonic kidney 293 cells (HEK293T) were purchased from Thermo Fisher Scientific (Waltham, MA, USA); two human fetal lung fibroblast cells (HFL-1 and MRC-5) and human breast adenocarcinoma cells (MCF-7) were purchased from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan). The H1299 human non-small cell lung cancer cell line was acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA). The HEK293T, HFL-1, MRC-5, and MCF-7 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (HyCloneTM, Cytiva, Marlborough, MA, USA) containing 10% fetal bovine serum (FBS; Sigma, St Louis, MO, USA) and 1% penicillin/streptomycin; the H1299 cell was cultured in RPMI (HyCloneTM, Cytiva, Marlborough, MA, USA) containing 10% FBS and 1% penicillin/streptomycin. All cell lines were incubated at 37 °C in a humidified incubator containing 5% CO2.
Hfl 1
Manufactured by RIKEN BioResource Center
Sourced in United States
The HFL-1 is a laboratory equipment product offered by RIKEN BioResource Center. It is designed to perform high-throughput liquid handling tasks. The core function of the HFL-1 is to automate the process of transferring small volumes of liquids between various containers, such as microplates, tubes, and reservoirs, with high precision and accuracy.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 7, by RIKEN BioResource Center (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Mrc 5, by RIKEN BioResource Center (1 mentions)
Hek293t, by Cytiva (1 mentions)
Hek293t, by Thermo Fisher Scientific (1 mentions)
Hyclonetm, by Cytiva (1 mentions)
H1299, by RIKEN BioResource Center (1 mentions)
2 protocols using hfl 1
1
Cell Culture Conditions for Cell Lines
2
Culturing Lung Cancer and Normal Fibroblast Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Non‐small cell lung cancer cell lines A549 and H1299 cells were obtained from Riken BRC (Tsukuba, Japan) and the American Type Culture Collection (Manassas, VA, USA), respectively. A549 and H1299 were grown in MEMα and RPMI‐1640, respectively, and supplemented with 10% (v/v) FBS. Normal human fibroblast cell line HFL1 cells were obtained from Riken BRC and were grown in MEMα supplemented with 15% FBS. All cells were maintained in a 37°C incubator with 5% CO2. Exponentially growing cells were used in the present study.
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