Log In Sign up
The largest database of trusted experimental protocols

Sc 67060

Manufactured by Santa Cruz Biotechnology
Visit Supplier

Sc-67060 is a lab equipment product offered by Santa Cruz Biotechnology. It is a centrifuge tube designed for use in various laboratory applications. The product's core function is to provide a secure and durable container for the handling and processing of samples during centrifugation.

Automatically generated - may contain errors

Lab products found in correlation

Ac 40, by Merck Group (2 mentions) Sc 816, by Santa Cruz Biotechnology (2 mentions) Sc 166394, by Santa Cruz Biotechnology (2 mentions) Sc 20681, by Santa Cruz Biotechnology (2 mentions) Ma5 13065, by Thermo Fisher Scientific (2 mentions) Ma1 310, by Thermo Fisher Scientific (2 mentions) Sc 32233, by Santa Cruz Biotechnology (2 mentions)

2 protocols using sc 67060

1

Protein Expression and Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
+ Open protocol
+ Expand
2

Protein Expression and Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!