LP contents in liver and egg yolk were extracted using the method of Boileau et al. (2000) (link) and analyzed by the method of Wei et al. (2001) (link) using high performance liquid chromatography (HPLC). In brief, approximately 0.1 g of liver tissue or egg yolk was homogenized thoroughly, dissolved in 6 ml of a potassium hydroxide/ethanol (1:5) solution containing 1 g/l butylated hydroxytoluene (BHT) and saponified at 60°C for 30 min. LP was extracted twice under yellow light using equal volumes of hexane (6ml) plus 2ml of distilled water. The extracts were dried and stored at −C20°C for no longer than 2 d before LP measurement by HPLC. The HPLC system included Waters 510 pumps, a Waters 717 plus auto sampler, a Waters 486 Tunable Absorbance detector, and Waters Nova-Pak (5 µm, 3.9 cm×300 mm) C18 column. The mobile phase was methanol:acetonitrile:chlorform (47:47:6, v/v/v) and the flow rate was 1.0 ml/min. The HPLC was controlled by Waters Millennium chromatography software and the lycopene peak was monitored at 472 nm. LP concentration was calculated using a calibration curve prepared with the pure LP standard (L-9879, Sigma Co., St. Louis, MO, USA).
L9879
Manufactured by Merck Group
Sourced in United States
The L9879 is a precision laboratory equipment designed for conducting various scientific experiments and analyses. It serves as a versatile and reliable tool for researchers and scientists in a wide range of fields. The core function of the L9879 is to provide accurate and consistent measurements, data collection, and sample processing capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Photodiode array detector, by Waters Corporation (2 mentions)
Empower 3, by Waters Corporation (2 mentions)
Acquity hss t3 75 2.1 mm 1 8 μm column, by Waters Corporation (2 mentions)
Acquity hss t3 1.8 μm vanguard precolumn, by Waters Corporation (2 mentions)
Waters 717 plus autosampler, by Waters Corporation (1 mentions)
Waters 486 tunable absorbance detector, by Waters Corporation (1 mentions)
Millennium chromatography software, by Waters Corporation (1 mentions)
Nova pak, by Waters Corporation (1 mentions)
Lycopene, by Merck Group (1 mentions)
4 protocols using l9879
1
Quantifying Lycopene in Liver and Egg
2
Lycopene Effects on Pancreatic Cancer
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PANC-1 cells (1–5 × 104/mL) were treated with a solution of lycopene (L9879, Sigma-Aldrich, St. Louis, MO, USA) in tetrahydrofuran (THF) to produce final lycopene concentrations of 0.25 or 0.5 μM. The mixtures were then incubated for 24 h to determine cell viability, the levels Bcl-2 and Bax, intracellular and mitochondrial ROS levels, OCR, DNA binding activity of NF-kB and mRNA and protein expression of IκBα, cIAP1, cIAP2, and survivin. Controls for the experiments were PANC-1 cells incubated with THF (less than 0.3%) alone.
3
Serum Lycopene Quantification by HPLC
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The lycopene concentration in all serum samples was measured in duplicate by high‐performance liquid chromatography (Diwadkar‐Navsariwala et al., 2003 ) with modifications. Briefly, 400 μl of serum was mixed with 400 μl of ethanol and was extracted twice with 2 ml hexane. The combined hexane layers were evaporated to dryness in a vacuum (Scan Speed 32 centrifuge) and the residue reconstituted to a volume of 100 μl in sample solution (absolute ethanol – methylene chloride, 5:1, v/v). The specimens were centrifuged again (15 min at 10,000 g), and clear supernatant was transferred to HPLC vials. Five microliters of the extract was injected into an Acquity HSS T3 75 × 2.1 mm 1.8 μm column (Waters, USA) preceded by an Acquity HSS T3 1.8 μm VanGuard precolumn (Waters) and eluted isocratically at 45°C with the mobile phase (acetonitrile—0.08% phosphoric acid solution—tert‐butyl methyl ether, 70:5:25, v/v/v) at a flow rate of 0.5 ml/min. The lycopene peak was detected by a Photodiode Array Detector (Waters) at 474 nm. The peak area was measured using Empower 3 software (Waters, MA). The lycopene concentration in serum samples was calculated by reference to an analytical standard (lycopene from tomato, L‐9879; Sigma, USA).
4
Quantifying Lycopene in Serum Samples
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Lycopene concentration in serum samples was measured using HPLC (Diwadkar-Navsariwala et al., 2003) with modifications. Only samples from volunteers receiving lycopene-enriched ice cream were analyzed. Briefly, 400 μl of serum was mixed with 400 μL of ethanol and extracted twice with 2 mL of hexane. The combined hexane layers were evaporated to dryness under vacuum (Scan Speed 32 centrifuge, Labogene, Copenhagen, Denmark), and the residue was reconstituted to 100 μL in sample solution (absolute ethanol: methylene chloride, 5:1 vol/vol). The specimens were centrifuged again (15 min at 10,000 × g at 4°C) and the supernatant was transferred to HPLC vials. The extract (5 μL) was injected into an Acquity HSS T3 75 × 2.1 mm, 1.8-μm column (Waters, Milford, MA) preceded by a Acquity HSS T3 1.8-μm VanGuard precolumn (Waters) and eluted isocratically at 45°C with the mobile phase (acetonitrile: 0 .08 % phosphoric acid solution: tert -Butyl methyl ether, 70:5:25 vol/vol/vol) at a flow rate of 0.5 mL/min. The peaks corresponding to lycopene isomers were detected by a Photodiode Array Detector (Waters) at 474 nm. The peak areas were measured using Empower 3 software (Waters). Lycopene concentrations were calculated by reference to an analytical standard (lycopene from tomato, L9879, Sigma-Aldrich, St. Louis, MO).
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