Tianamp viral genomic dna rna kit

Using the DIG high prime DNA labeling and detection starter kit Ⅱ (11585614910; Roche Diagnostics GmbH,German), and the pEASY-HBV plasmid as a template, the HBV Southern blot probe was prepared. DNA was extracted from mouse liver tissue using the TIANamp viral genomic DNA/RNA Kit as described in the instructions(YDP315; Tiangen Biochemical Technology, Beijing, China). Briefly, DNA was isolated in 1.2 % agarsugar gel, then transferred to a nylon membrane (11417240001, Roche, Switzerland), incubated overnight with a synthetic probe, then incubated overnight with digoxin antibodies, and used developer (6610190; Carestream, Canada) to develop. Southern bolt marker is provided by Prof. Ni Tang from Chongqing Medical University.

Total DNA was extracted from cells and mouse liver tissues using the TIANamp Genomic DNA Kit as described in the instructions (DP304; Tiangen Biochemical Technology, Beijing, China). Total DNA was extracted from mouse serum using the TIANamp viral genomic DNA/RNA Kit as described in the instructions (YDP315; Tiangen Biochemical Technology, Beijing, China). Total RNA was extracted from cells and mouse liver tissues using the RNA Quick Purification Kit as described in the instructions (RN001; ES Science, Shanghai, China). RNA was reverse-transcribed into cDNA using RT Master Mix for qPCR II as described in the instructions (HY-K0511A; MedChemExpress, Monmouth Junction, NJ, USA).