Total RNA was isolated and first strand cDNA was synthesized using iScript (Bio-Rad). Quantitative real-time PCR with a BioRad iCycler® used primers mixed with the iQ™SYBR®Green Supermix (Bio-Rad). Primer sets for Vegfr1, Vegfr2, Tie2, Itg1, Vegf-variants, Mmp2, Mmp9, Timp1, Timp2, Timp3, Col1a1, Col6a5, Col6a3, Col6a1/2, Col18a1, Col4a1, Lama5 and Gapdh were purchased from Invitrogen. Primers are listed in Supplementary Table 1 . Specificity of reactions was determined by melting curve analysis. The relative fold changes of gene expression between each gene of interest were calculated by standard curve method. For each gene of interest ≥3 independent experiments were performed and each experiment was run in duplicates or triplicates.
Col6a5
Manufactured by Thermo Fisher Scientific
Col6a5 is a protein-coding gene that provides instructions for making a component of type VI collagen, a structural protein found in the extracellular matrix of various tissues. The primary function of Col6a5 is to contribute to the formation and stability of collagen VI fibrils.
Automatically generated - may contain errors
Lab products found in correlation
Iq sybr green supermix, by Bio-Rad (2 mentions)
Icycler, by Bio-Rad (2 mentions)
Gapdh, by Thermo Fisher Scientific (2 mentions)
Col6a3, by Thermo Fisher Scientific (2 mentions)
Col4a1, by Thermo Fisher Scientific (2 mentions)
Iscript, by Bio-Rad (2 mentions)
Col18a1, by Thermo Fisher Scientific (2 mentions)
2 protocols using col6a5
1
Quantitative Real-Time PCR Analysis
2
Quantitative Real-Time PCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated and first strand cDNA was synthesized using iScript (Bio-Rad). Quantitative real-time PCR with a BioRad iCycler® used primers mixed with the iQ™SYBR®Green Supermix (Bio-Rad). Primer sets for Vegfr1, Vegfr2, Tie2, Itg1, Vegf-variants, Mmp2, Mmp9, Timp1, Timp2, Timp3, Col1a1, Col6a5, Col6a3, Col6a1/2, Col18a1, Col4a1, Lama5 and Gapdh were purchased from Invitrogen. Primers are listed in Supplementary Table 1 . Specificity of reactions was determined by melting curve analysis. The relative fold changes of gene expression between each gene of interest were calculated by standard curve method. For each gene of interest ≥3 independent experiments were performed and each experiment was run in duplicates or triplicates.
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