Anti cd19 j3 119

At the indicated time points, inguinal lymph nodes were collected and flash frozen. GI biopsies from upper GI (duodenum) and lower GI (colon) were collected as described previously.²⁹ (link) Single-cell suspensions were prepared from cell culture or peripheral tissues. Peripheral blood cell subsets were sorted using magnetic bead kits from Miltenyi Biotec (Bergisch Gladbach, Germany) or through antibody labeling and a FACSAria II machine (BD Biosciences, Franklin Lakes, NJ, USA). The following antibodies (clones) were purchased from BD Biosciences: CD3 (SP34-2), CD4 (L200), CD45 (D058-1283), CCR5 (3A9), CD11b (ICRF44), CD14 (M5E2), CD16 (3G8), CD34 (563), CD28 (CD28.2), and CD95 (DX2). Anti-CD8 (B9.11) and anti-CD19 (J3-119) were purchased from Beckman Coulter (Brea, CA, USA). Anti-CD38 (AT-1) was purchased from STEMCELL Technologies (Vancouver, BC, Canada). For surface marker measurement, individual antibodies were added to the cell suspension alone or together. Isotype control antibodies were used for gating the positive group of target cells. Cells were stained with antibodies for 20 min at room temperature followed by washing twice with PBS plus 2% FBS. After resuspension, cells were subjected to FACS analysis in less than 8 h.