The main reagents are the following: Pregnant mare serum gonadotropin (PMSG) (2nd Ningbo Hormone Production Company, Zhejiang, China), Human chorionic gonadotropin (hCG) (Prospec, Israel), M2 medium, ICA, H2O2 solution and DMSO (Sigma, USA), KSOM medium (Millipore, Germany), TRNzol (Tiangen, Beijing, China), Quick-RNA™ MicroPrep and SYBR® Premix Ex Taq™ (Roche, Swiss), Reverse Transcription Kit and dNTP Mix (Thermo, USA), DAPI and JC-1 (Beyotime, China), and DCFH-DA (Molecular probes, USA).
Reverse transcription kit and dntp mix
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Reverse Transcription Kit and dNTP Mix are laboratory reagents used for the conversion of RNA into complementary DNA (cDNA) through the process of reverse transcription. The kit includes the necessary enzymes, buffers, and dNTP (deoxynucleotide triphosphate) mix required to perform this fundamental step in many molecular biology and genomics applications.
Automatically generated - may contain errors
Lab products found in correlation
Ksom medium, by Merck Group (2 mentions)
Trnzol, by Tiangen Biotech (1 mentions)
Dcfh da, by Thermo Fisher Scientific (1 mentions)
M2 medium, by Merck Group (1 mentions)
H2o2 solution, by Merck Group (1 mentions)
Human chorionic gonadotropin hcg, by ProSpec (1 mentions)
Sybr premix ex taqtm, by Roche (1 mentions)
Tyrode solution, by Merck Group (1 mentions)
Leica tcs sp5 confocal laser scanning microscope, by Leica Microsystems (1 mentions)
Zr rna microprep kit, by Zymo Research (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
2 protocols using reverse transcription kit and dntp mix
1
Oocyte Maturation and Embryo Development
2
Characterization of Embryonic Stem Cell Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human chorionic gonadotropin (hCG) (Prospec Company, Israel). Pregnant mare serum gonadotropin (PMSG) (2nd Ningbo Hormone Producion Company, China). M2 medium, Dimethyl sulfoxide (DMSO) and Tyrode solution (Sigma, USA). KSOM medium (Millipore, USA). MPP and PPT (Tocris Bioscience, UK). DAPI (Beyotime Institute of Biotechnology, China). Alexa Fluor 594 labeled donkey anti-rabbit secondary antibody and Alexa Fluor 488 labeled donkey antimouse secondary antibody (Life Technology, USA). ZR RNA MicroPrep kit (Zymo, USA). Reverse Transcription Kit and dNTP Mix (ThermoScientific, USA). SYBR Premix Ex TaqTM (Roche, Swiss). Inverted microscope (Nikon, Japan). The confocal images were taken with a Leica TCS SP5 laser scanning confocal microscope from Leica Microsystems, Germany. Real-time PCR was performed with PikoReal 2.2.248 TCR0096 PCR instrument from ThermoScientific, USA.
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