After completion of LSI, sham-operated and ischemic Malat1 WT and KO mice (n = 5 per group) were perfused with saline, and the gastrocnemius muscles were dissected out from both ischemic and contralateral hindlimb. Coronal sections (8 µm) were cryosectioned for immunostaining with rat anti-CD31 (BD Pharmingen, San Diego, CA, USA 1:200) or rabbit anti-VEGFR2 antibody (Cell Signaling Technology, Danvers, MA, USA, 1:200). Secondary antibodies: Cy3-conjugated goat anti-rat IgG, Alexa Fluor 488-conjugated goat anti-rabbit IgG (all at 1:1000; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA). Images were collected on confocal microscope (FV1000-II; Olympus; Tokyo, Japan) and processed in Adobe Photoshop (CS2 version 9.0, Adobe Systems, San Jose, CA, USA) for compositions [56 (link)].
Rabbit anti vegfr2 antibody
Manufactured by Cell Signaling Technology
Sourced in United States, United Kingdom
The Rabbit anti-VEGFR2 antibody is a reagent used to detect the presence and expression levels of the Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) protein in biological samples. This antibody is raised in rabbits and can be used in various immunodetection techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the VEGFR2 signaling pathway.
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Lab products found in correlation
Cy3 conjugated goat anti rat igg, by Jackson ImmunoResearch (1 mentions)
Rat anti cd31, by BD (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Fv1000 2, by Olympus (1 mentions)
Rabbit anti p erk, by Cell Signaling Technology (1 mentions)
Goat anti mouse endocan antibody, by R&D Systems (1 mentions)
Rabbit anti p jnk antibody, by Cell Signaling Technology (1 mentions)
Mmp 9 antibody, by Cell Signaling Technology (1 mentions)
Rabbit anti p38 antibody, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit anti vegfr2 antibody
1
Measuring Angiogenesis in Ischemic Muscle
2
Molecular Profiling of Ocular Angiogenesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fresh isolated eye tissues and cells were lysed, quantified, blotted and developed as previously described. 18 The following primary antibodies were used for the purpose of this experiment: goat anti-mouse endocan antibody (0.1 lg/mL; R&D Systems); rabbit anti-matrix metalloproteinase (MMP)-2 antibody (1:1000; Cell Signaling Technology); MMP-9 antibody (1:1000; Cell Signaling Technology); rabbit anti-PIGF antibody (1:1000; Abcam, Cambridge, UK); goat anti-VEGF antibody (1:1000; Abcam); rabbit anti-VEGFR1 antibody (1:1000; Abcam); rabbit anti-VEGFR2 antibody (Cell Signaling Technology); rabbit anti-P38 antibody (1:1000; Cell Signaling Technology); rabbit anti-phospho (p)-P38 antibody (1:1000; Cell Signaling Technology); rabbit anti-extracellular signal-regulated kinase (ERK) antibody (1:1000; Cell Signaling Technology); rabbit anti-p-ERK (1:1000; Cell Signaling Technology); rabbit anti-c-Jun Nterminal kinases (JNK) antibody (1:1000; Cell Signaling Technology) and rabbit anti-p-JNK antibody (1:1000; Cell Signaling
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