Immunohischemical staining for smooth muscle cell α-actin, von Willebrand factor (vWF), and leukocyte specific markers was performed on frozen sections of the carotid arteries using the following primary antibodies: Mouse anti-human α-smooth muscle actin IgG (Dako Corp.); rat anti-mouse macrophage/monocyte IgG, clone MOMA-2 (Serotec); rabbit anti-human von Willebrand factor (vWF) (Sigma); rat anti-mouse CD4 antibody (Millipore); rabbit anti-mouse CD8 IgG (Cell Signaling); rat anti-mouse Ly-6G antibody (eBioscience); and FITC anti-mouse Ly-6G IgG (BD Biosciences). Subsequent incubations with biotinylated secondary antibody and VECTASTAIN Elite ABC HRP Kit (Vector Laboratories) were performed as previously described [41 (link)]. Sections stained with fluorescent-labeled antibody or DAPI fluoromount-G (Southern Biotech) were directly visualized with a fluorescence microscope.
Rat anti mouse cd4 antibody
Manufactured by Merck Group
The Rat anti-mouse CD4 antibody is a laboratory reagent used in immunological research. It is a monoclonal antibody that specifically binds to the CD4 protein expressed on the surface of certain T lymphocytes in mice. This antibody can be used to identify and study these CD4-positive T cells in various experimental settings.
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Lab products found in correlation
Dapi fluoromount g, by Southern Biotech (2 mentions)
Rat anti mouse ly 6g antibody, by Thermo Fisher Scientific (2 mentions)
Vectastain elite abc hrp kit, by Vector Laboratories (1 mentions)
Vectastain elite abc kit, by Vector Laboratories (1 mentions)
Rabbit anti mouse cd8 igg d4w2z, by Cell Signaling Technology (1 mentions)
2 protocols using rat anti mouse cd4 antibody
1
Immunohistochemical Profiling of Carotid Arteries
2
Immunostaining Protocol for Atherosclerosis
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Immunostaining was performed on frozen sections using the following primary antibodies: rat anti‐mouse Ly6G antibody (eBioscience), rat anti‐mouse CD4 antibody (Millipore), rat anti‐mouse macrophage/monocyte IgG, clone MOMA‐2 (Serotec), goat anti‐α‐smooth muscle actin antibody (Arigo Biolaboratories), rat anti‐mouse macrophage/monocyte IgG, clone M3/84(BD Biosciences), rabbit anti‐mouse CD8 IgG (D4W2Z, Cell Signaling), goat anti‐mouse IgG (MJE00B, R&D Systems), and polyclonal goat anti‐mouse N‐formyl peptide receptor (FPR) (Santa Cruz Biotech) and MCP‐1 antibodies (R&D System). Immunoreactivity was visualized with the Vectastain Elite ABC Kit (Vector Laboratories). Sections stained with Alexa Fluor 488‐conjugated goat anti‐rat IgG (H+L) (4416S, Cell Signaling), NL557‐conjugated donkey anti‐goat IgG (H+L) (NL001, R&D Systems), or DAPI fluoromount‐G (0100–20, Southern Biotech) were directly visualized by fluorescence microscopy.
The density of macrophages, smooth muscle cells, and total cells in primary atherosclerosis was quantified on immunofluorescent‐stained sections for three or five mice per strain. Cells in two different fields at ×40 magnification were counted for each section. Selected sections of ligated arteries were immunostained for inflammatory cells, smooth muscle cells, and MCP‐1.
The density of macrophages, smooth muscle cells, and total cells in primary atherosclerosis was quantified on immunofluorescent‐stained sections for three or five mice per strain. Cells in two different fields at ×40 magnification were counted for each section. Selected sections of ligated arteries were immunostained for inflammatory cells, smooth muscle cells, and MCP‐1.
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