The same criteria were used for mouse handling as described above. Prior
to experiments, fiberoptic cannulas implanted into the PBNL were
attached through zirconia sleeves (Doric Lenses) to branching fiberoptic
patchcords (Doric Lenses) connected to a blue LED (Doric Lenses) and a
programmable LED driver (Doric Lenses). Optogenetic stimulation was controlled
by the combination of custom programs written in Bonsai software and Doric
Neuroscience Studio (4.1.5.2) through an Arduino circuit board (Uno, Arduino)
and custom sketches written in Arduino software (1.8.7). Approximately the
initial half of the experiments were performed and analyzed by investigators who
were not blinded to genotype, and the remainder of the experiments were repeated
by investigators who were blinded to genotype.
to experiments, fiberoptic cannulas implanted into the PBNL were
attached through zirconia sleeves (Doric Lenses) to branching fiberoptic
patchcords (Doric Lenses) connected to a blue LED (Doric Lenses) and a
programmable LED driver (Doric Lenses). Optogenetic stimulation was controlled
by the combination of custom programs written in Bonsai software and Doric
Neuroscience Studio (4.1.5.2) through an Arduino circuit board (Uno, Arduino)
and custom sketches written in Arduino software (1.8.7). Approximately the
initial half of the experiments were performed and analyzed by investigators who
were not blinded to genotype, and the remainder of the experiments were repeated
by investigators who were blinded to genotype.