Cardiac blood was collected from animals at week 7 for cytokine analyses. Serum levels of C-peptide Cloudclone (CEA447Ra, Uscn Life Science Inc. Wuhan, China), insulin (CEA448Ra, Uscn Life Science Inc. Wuhan, China), Th1-related IFN-γ (SEA049Ra, Uscn Life Science Inc. Wuhan, China), Th17-related IL-17 (SEA063Ra, Uscn Life Science Inc. Wuhan, China) as pro-inflammatory, and Th2-related IL-4 (SEA063Ra, Uscn Life Science Inc. Wuhan, China) and Treg-related IL-10 (SEA056Ra, Uscn Life Science Inc. Wuhan, China) cytokines as anti-inflammatory were evaluated by ELISA analysis. The manufacturer’s instructions were followed during the analyses, and the measurements were made with a 450-nm microplate reader (Allsheng AMR-100 Microplate Reader AS-16050-00). With the measured absorbance values, the concentrations of the samples were calculated as pg/ml.
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