Anti setdb1

The following chemicals and concentrations were used: Human recombinant VEGF-A 165 (R&D, 293-VE), Recombinant Human FGF-basic (154 a.a.) (bFGF, Peprotech, 100-18B), RNase A (NEB, EN0531), RNase H (NEB, M0297L), Cycloheximide (Sigma, C1988) and human recombinant TNF-α (Peprotech, 300-01 A). The following antibodies were used: Anti-H3-pan (Diagenode, C15200011), Anti-dsDNA [35I9 DNA] (Abcam, ab27156), Anti-DNA-RNA Hybrid [S9.6] (Kerafast, ENH001), Anti-EPHA2 (Bethyl, A302-025-M), Anti-GAPDH (Sigma, G8795), Anti-HSC70/HSP70 (Enzo Life Sciences, ADI-SPA-820), Anti-NONO (Bethyl, A300-587A), Anti-MPP8 (Bethyl, A303-051A-M), Recombinant Anti-6X His tag® antibody [EPR20547] (Abcam, ab213204, ChIP grade), Anti-H3K9me3 (Diagenode, SN-146-100), Anti-SETDB1 (Bethyl, A300-121A, for chromatin immunoprecipitation; Santa Cruz Biotechnology, ESET (G-4): sc-271488, for Proximity ligation assay) and Anti-ZNF638/NP220 (Bethyl, A301-548A-M).

The following antibodies were used: anti-ERβ (L-20), anti-ERα (HC-20) and anti-AR (C-19) from Santa Cruz Biotechnology, Dallas US-TX; anti-ERβ from GeneTex, Irvine, CA; anti-eNOS from BD Biosciences, Franklin Lakes, US-NJ; anti-phospo-eNOS (Ser1177) from Cell Signaling, Danvers, US-MA; anti-AR (PG-21) from Upstate, Lake Placid, US-NY; anti-ERα Ab-10 (TE111.5D11) from Thermo Fisher Scientific, Fremont, US-CA; anti-KDM1A (also named LSD1), anti-H3K9me3, anti-H3K9me2 and anti-H3K27me3 from Active Motif, Carlsbad, US-CA; anti-H3K9me2 from Epigentek, Farmingdale, US-NY; anti-CBX4 (also named Pc2), anti-KDM1A and anti-SETDB1 from Bethyl Laboratories, Montgomery US-TX; anti-KDM4D (also named JMJD2D) from Novus Biological, Littleton US-CO; anti-EZH2 from Abcam, Cambridge UK; anti-HSP70 from StressGen Biotechnologies, San Diego US-CA.