Western blotting was performed as described previously (22 (link)). The used antibodies were as follows: anti-LC3 (Cell Signaling Technology, 3868), anti-p62 (Proteintech, 18420-1-AP), anti-mTOR (Cell Signaling Technology, 2972), anti-phospho-mTOR (Cell Signaling Technology, 2971), anti-ULK1 (Cell Signaling Technology, 8054), anti-phospho-ULK1 (Ser757) (Cell Signaling Technology, 6888), anti-VPS34 (Proteintech, 12452-1-AP), anti-Beclin-1 (Proteintech, 11306-1-AP) and anti-β-actin (Santa Cruz Biotechnology, sc-47778). Anti-VP1, a rabbit polyclonal antibody, was produced by Wuhan Abclonal Biotechnology. The relative band intensities of the proteins were quantified using the NIH ImageJ software.
Anti vps34
Manufactured by Proteintech
Sourced in United States
Anti-Vps34 is a laboratory reagent that binds to and inhibits the function of the Vps34 protein. Vps34 is a class III phosphoinositide 3-kinase that plays a crucial role in various cellular processes, including autophagy and endocytic trafficking. This reagent can be used in research applications to study the biological functions of Vps34 and its involvement in cellular pathways.
Automatically generated - may contain errors
Lab products found in correlation
Lomitapide, by Selleck Chemicals (2 mentions)
Anti phospho beclin 1 ser93, by Cell Signaling Technology (2 mentions)
Glutathione s transferase tag protein puri cation kit, by Beyotime (2 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Anti beclin 1, by Cell Signaling Technology (2 mentions)
Anti ampkα, by Cell Signaling Technology (2 mentions)
Anti phospho ampkα thr172, by Cell Signaling Technology (2 mentions)
Prescission protease, by Beyotime (2 mentions)
Anti atg14, by Proteintech (2 mentions)
Protein a g plus agarose, by Santa Cruz Biotechnology (2 mentions)
Anti lc3b, by Cell Signaling Technology (2 mentions)
Anti cleaved parp, by Cell Signaling Technology (2 mentions)
Anti caspase 3, by Cell Signaling Technology (2 mentions)
Anti gapdh, by Proteintech (2 mentions)
Reactive oxygen species assay kit, by Beyotime (2 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Keygen Biotech (2 mentions)
Wst 1 cell proliferation and cytotoxicity assay kit, by Beyotime (2 mentions)
Anti phospho ulk1 ser757, by Cell Signaling Technology (1 mentions)
Anti phospho mtor, by Cell Signaling Technology (1 mentions)
Anti ulk1, by Cell Signaling Technology (1 mentions)
3 protocols using anti vps34
1
Quantitative Western Blot Analysis
2
Lomitapide Apoptosis and Autophagy Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Drugs, antibodies and reagents Lomitapide was acquired from Selleck Chemicals (Huston, TX, USA) and freshly prepared into 5 mM stock solution with DMSO. Anti-Caspase-3, anti-cleaved caspase-3, anti-cleaved PARP, anti-LC3B, anti-AMPKα, anti-phospho-AMPKα Thr172, anti-phospho-Beclin-1 Ser93, and anti-Beclin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Vps34, anti-Atg14 and anti-Gapdh antibodies were obtained from Proteintech (Chicago, IL, USA). The WST-1 cell proliferation and cytotoxicity assay kit, glutathione S-transferase (GST) tag protein puri cation kit, reactive oxygen species assay kit and PreScission protease were obtained from Beyotime Biotechnology (Shanghai, China). An Annexin V-FITC/PI apoptosis detection kit was obtained from KeyGen Biotechnology (Nanjing, Jiangsu, China). Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
3
Lomitapide Apoptosis and Autophagy Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Drugs, antibodies and reagents Lomitapide was acquired from Selleck Chemicals (Huston, TX, USA) and freshly prepared into 5 mM stock solution with DMSO. Anti-Caspase-3, anti-cleaved caspase-3, anti-cleaved PARP, anti-LC3B, anti-AMPKα, anti-phospho-AMPKα Thr172, anti-phospho-Beclin-1 Ser93, and anti-Beclin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Vps34, anti-Atg14 and anti-Gapdh antibodies were obtained from Proteintech (Chicago, IL, USA). The WST-1 cell proliferation and cytotoxicity assay kit, glutathione S-transferase (GST) tag protein puri cation kit, reactive oxygen species assay kit and PreScission protease were obtained from Beyotime Biotechnology (Shanghai, China). An Annexin V-FITC/PI apoptosis detection kit was obtained from KeyGen Biotechnology (Nanjing, Jiangsu, China). Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
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