Antigen retrieval was performed by autoclave treatment at 100℃ for 10 min using Target retrieval solution High P.H. (DAKO Cytomation, Kyoto, Japan). At rst, GST-pi polyclonal antibody (MBL, Nagoya, Japan), COX-2 polyclonal antibody (Cayman Chemical, Chicago, IL), P53 polyclonal antibody (FL393) (Santa Cruz, Santacruz, CA), and Smad4 monoclonal antibody (B-8) (Santa Cruz) was used. Proteins were identi ed with Envision kit (DAKO cytomation, Kyoto, Japan). They were colored by 3,3-Diaminobenzidine Tetrahydrochloride (DAB) (WAKO, Osaka, Japan), and their nuclei were stained by Gill's hematoxylin solution (WAKO). It was determined to be positive when more than 50% of the composing cells was stained.
Smad4 monoclonal antibody b 8
Manufactured by Santa Cruz Biotechnology
The Smad4 monoclonal antibody (B-8) is a laboratory tool used to detect and study the Smad4 protein. Smad4 is a key component in the transforming growth factor-beta (TGF-β) signaling pathway, which plays a crucial role in various cellular processes. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to identify and analyze the Smad4 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
P53 polyclonal antibody fl393, by Santa Cruz Biotechnology (2 mentions)
Cox 2 polyclonal antibody, by Cayman Chemical (2 mentions)
3 3 diaminobenzidine tetrahydrochloride dab, by Fujifilm (2 mentions)
Gill s hematoxylin solution, by Fujifilm (2 mentions)
Envision kit, by Agilent Technologies (2 mentions)
2 protocols using smad4 monoclonal antibody b 8
1
Immunohistochemical Analysis of Protein Expression
2
Immunohistochemical Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antigen retrieval was performed by autoclave treatment at 100℃ for 10 min using Target retrieval solution High P.H. (DAKO Cytomation, Kyoto, Japan). At rst, GST-pi polyclonal antibody (MBL, Nagoya, Japan), COX-2 polyclonal antibody (Cayman Chemical, Chicago, IL), P53 polyclonal antibody (FL393) (Santa Cruz, Santacruz, CA), and Smad4 monoclonal antibody (B-8) (Santa Cruz) was used. Proteins were identi ed with Envision kit (DAKO cytomation, Kyoto, Japan). They were colored by 3,3-Diaminobenzidine Tetrahydrochloride (DAB) (WAKO, Osaka, Japan), and their nuclei were stained by Gill's hematoxylin solution (WAKO). It was determined to be positive when more than 50% of the composing cells was stained.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!